High doses of antimicrobial agents are a huge threat due to the increasing number of pathogenic organisms that are becoming resistant to antimicrobial agents. This resistance has led to a search for alternatives. Therefore, this study presents the synthesis and characterization of ZrO₂-Ag₂O nanoparticles (NPs) by sol-gel. The NPs were analyzed by dynamic light scattering (DLS), UV-visible (UV-vis), Raman and scanning electron microscopy (SEM). The NPs were later evaluated for their antifungal effects against Candidaalbicans, Candida dubliniensis, Candida glabrata, and Candida tropicalis, using disc diffusion and microdilution methods, followed by the viability study. The DLS showed sizes for ZrO₂ 76 nm, Ag₂O 50 nm, and ZrO₂-Ag₂O samples between 14 and 42 nm. UV-vis shows an absorption peak at 300 nm for ZrO₂ and a broadband for Ag₂O NPs. Raman spectra were consistent with factor group analysis predictions. SEM showed spherically shaped NPs. The antifungal activity result suggested that ZrO₂-Ag₂O NPs were effective against Candida spp. From the viability study, there was no significance difference in viability as a function of time and concentration on human mononuclear cells. This promising result can contribute toward the development of alternative therapies to treat fungal diseases in humans.

中文翻译：

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混合氧化物金属纳米复合材料对念珠菌的抗真菌活性评估。

高剂量的抗微生物剂是巨大的威胁，这是由于越来越多的对抗微生物剂产生耐药性的病原性生物体所致。这种抵制导致人们寻找替代品。因此，本研究提出了溶胶-凝胶法合成ZrO ₂ -Ag ₂ O纳米粒子（NPs）和表征。通过动态光散射（DLS），紫外可见光（UV-vis），拉曼光谱和扫描电子显微镜（SEM）分析NP。该纳米粒子后来被评估它们对防霉效果念珠菌白色念珠菌，都柏林念珠菌，光滑念珠菌，和热带念珠菌，使用圆盘扩散法和微量稀释法，然后进行可行性研究。DLS显示14至42 nm之间的ZrO ₂ 76 nm，Ag ₂ O 50 nm和ZrO ₂ -Ag ₂ O样品的尺寸。UV-vis显示ZrO ₂在300 nm处的吸收峰和Ag ₂ O NPs在宽带处的吸收峰。拉曼光谱与因子组分析预测一致。SEM显示球形NP。抗真菌活性结果表明，ZrO ₂ -Ag ₂ O NPs对念珠菌有效。spp。从活力研究来看，在人类单核细胞上，活力随时间和浓度的变化没有显着差异。这一有希望的结果可以有助于开发替代疗法来治疗人类的真菌疾病。