Abstract

Point of care testing makes it possible to obtain results in an extremely short time. Recently, radiometer has expanded the panel of tests available on its ABL90 FLEX PLUS blood gas analyzer (ABL90) by adding urea and creatinine. The aim of this study was to verify the performance of these new parameters. This included assessment of imprecision, linearity, accuracy by comparison with central laboratory standard assays and interferences. In addition, clinical utility in a dialysis center was evaluated. Within-lab coefficients of variation were close to 2%. The mean and limits of agreement (mean ± 1.96 SD) of the difference between ABL90 and Roche enzymatic assays on cobas 8000 were 0.5 (from −1.4 to 2.3) mmol/L and −0.9 (from −19.5 to 17.8) µmol/L for urea and creatinine, respectively. The ABL90 enzymatic urea and creatinine assays met the acceptance criteria based on biological variation for imprecision and showed good agreement with central laboratory. The two assays were unaffected by hematocrit variation between 20 and 70%, hemolysis and icterus interferences. It should be noted that the relationship between lab methods and ABL90 was conserved even for high pre-dialysis values allowing easy access to dialysis adequacy parameters (Kt/V) and muscle mass evaluation (creatinine index). Rapid measurement of creatinine and urea using whole blood specimens on ABL90 appears as a fast and convenient method. Analytical performances were in accordance with our expectations without any significant interferences by hemolysis or icterus.

摘要

护理点测试可以在极短的时间内获得结果。最近，辐射计通过添加尿素和肌酐，扩展了其 ABL90 FLEX PLUS 血气分析仪 (ABL90) 上可用的测试范围。本研究的目的是验证这些新参数的性能。这包括通过与中心实验室标准测定和干扰进行比较来评估不精密度、线性度、准确性。此外，还评估了透析中心的临床效用。实验室内变异系数接近 2%。在 cobas 8000 上 ABL90 和 Roche 酶法测定之间差异的平均值和一致性极限（平均值 ± 1.96 SD）分别为 0.5（从 -1.4 到 2.3）mmol/L 和 -0.9（从 -19.5 到 17.8）μmol/L尿素和肌酐，分别。ABL90 酶促尿素和肌酐测定符合基于生物变异的不精确性验收标准，并与中心实验室表现出良好的一致性。两种测定不受 20% 至 70% 之间的血细胞比容变化、溶血和黄疸干扰的影响。应该注意的是，即使对于高透析前值，实验室方法和 ABL90 之间的关系也是保守的，从而可以轻松访问透析充分性参数（Kt / V）和肌肉质量评估（肌酐指数）。在 ABL90 上使用全血样本快速测量肌酐和尿素似乎是一种快速方便的方法。分析性能符合我们的预期，没有受到溶血或黄疸的任何显着干扰。