The genetic erosion of the Ananas Mill. genus has been accelerating in recent years. In the Pineapple Active Germplasm Bank (AGB) of the Embrapa Cassava and Fruits research unit (Embrapa Mandioca e Fruticultura), in vitro safety duplication has been an efficient strategy to protect this important germplasm. However, there are indications that in vitro culture conditions can cause somaclonal variations that interfere in the rescue and subsequent cultivation of the genetic material. Therefore, the objective of this work was to evaluate in field conditions the genetic stability of 45 accessions of different botanical varieties of Ananas, after in vitro conservation for 10 years, and to consolidate the efficiency of this type of conservation. Six plants of each accession were acclimatized and submitted to all the steps until transplantation to the field, where the morphological characterization was performed based on 21 quantitative and qualitative descriptors in two production cycles of pineapple plants. The accessions presented a normal production cycle, with genetic variations only noted in accessions AGB-003 and AGB-020 (Ananas comosus var. bracteatus (Lindl.) Coppens & F. Leal]). Some accessions in this evaluation could not be grouped with their corresponding plants grown in the AGB field, mainly regarding quantitative data. This was due to the greater vigor of these plants because they came from in vitro culture and were virus free. The results demonstrated the morphological stability of the preserved accessions even after the long in vitro conservation period, permitting the validation of this technique as a secure and efficient strategy to preserve pineapple germplasm.

凤梨工厂的遗传侵蚀。近年来，该属一直在加速。在Embrapa木薯和水果研究部门（Embrapa Mandioca e Fruticultura）的菠萝活性种质库（AGB）中，体外安全复制一直是保护这一重要种质的有效策略。但是，有迹象表明，体外培养条件可能会导致体细胞克隆变异，从而干扰遗传物质的抢救和后续培养。因此，这项工作的目的是在田间条件下评估凤梨不同植物品种的45种材料的遗传稳定性。，经过体外保存10年，并巩固了这类保存的效率。使每个登录的六种植物适应环境，并进行所有步骤，直到移植到田间，在此基础上，在两个菠萝生产周期中，基于21个定量和定性描述符对形态进行了表征。该种质提出了一个正常的生产周期，只在种质指出遗传变异AGB-003和AGB-020（菠萝变种bracteatus（Lindl。）Coppens和F. Leal]）。该评估中的一些入选物不能与在AGB领域中生长的相应植物分组，主要是关于定量数据。这是由于这些植物具有更大的活力，因为它们来自体外培养并且不含病毒。结果表明，即使经过很长的体外保存期，保存的种质仍能保持形态稳定，从而使该技术成为保存菠萝种质的安全有效策略的验证。