This study evaluated the anaesthetic and sedative effects of eugenol applied on yellow catfish (Pelteobagrus fulvidraco). In the anaesthesia induction and recovery experiment, fish were treated with five concentrations of eugenol (0, 15, 20, 25 and 30 mg/L), respectively, and in the transportation experiment, fish treated with 10 mg/L eugenol were evaluated after 12 h simulated transportation. Induction and recovery time were recorded. The induction time shortened with the increasing eugenol concentration, whereas the recovery time prolonged with the increasing eugenol concentration. The effective concentration of eugenol for short-term anaesthesia induction was between 25 and 30 mg/L. The post-transportation survival rate of yellow catfish was 100%. The content of ammonia nitrogen in the eugenol group was lower (p < 0.05). Compared with the control group, yellow catfish in the anaesthetized transportation group showed a significant decrease in blood cortisol levels (p < 0.05), whereas the levels of glucose and lysozyme significantly increased (p < 0.05). Both hsp70 and hsp90 mRNA expression in the liver of anaesthetized yellow catfish were found to significantly upregulate after transportation (p < 0.05). In summary, eugenol was an effective anaesthetic for yellow catfish. For short-term anaesthesia, the recommended concentration is 25–30 mg/L and for long-time transportation, the appropriate concentration is 10 mg/L in order to reduce stress response of fish.

中文翻译：

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丁香酚对黄鲇（Pelteobagrus fulvidraco）保存和运输的麻醉作用

本研究评估了丁香酚对黄鲶（Pelteobagrus fulvidraco）的麻醉和镇静作用。）。在麻醉诱导和恢复实验中，分别用五种浓度的丁香酚（0、15、20、25和30mg/L）处理鱼，在运输实验中，对用10mg/L丁香酚处理的鱼进行评估。 12 小时模拟运输。记录诱导和恢复时间。诱导时间随着丁香酚浓度的增加而缩短，而恢复时间随着丁香酚浓度的增加而延长。丁香酚短期麻醉诱导的有效浓度为25～30mg/L。黄颡鱼运输后成活率为100%。丁香酚组氨氮含量较低（p < 0.05）。与对照组相比，麻醉转运组黄鲇鱼血皮质醇水平显着降低（p <  0.05），而葡萄糖和溶菌酶水平显着升高（p <  0.05）。既HSP70和HSP90在麻醉黄色鲶鱼的肝脏mRNA的表达运输（后发现显著上调P <  0.05）。总之，丁香酚是黄鲶的有效麻醉剂。对于短期麻醉，推荐浓度为25-30mg/L，对于长时间运输，适宜浓度为10mg/L，以减少鱼类的应激反应。