Optical chemosensors for the detection of proximally phosphorylated peptides and proteins,RSC Chemical Biology

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Optical chemosensors for the detection of proximally phosphorylated peptides and proteins
RSC Chemical Biology ( IF 4.2 ) Pub Date : 2021-4-21 , DOI: 10.1039/d1cb00055a
Aaron D Cabral _{1,

2} , Tudor B Radu _{1,

2} , Elvin D de Araujo ₁ , Patrick T Gunning _{1,

2}

Affiliation

Proximal multi-site phosphorylation is a critical post-translational modification in protein biology. The additive effects of multiple phosphosite clusters in close spatial proximity triggers integrative and cooperative effects on protein conformation and activity. Proximal phosphorylation has been shown to modulate signal transduction pathways and gene expression, and as a result, is implicated in a broad range of disease states through altered protein function and/or localization including enzyme overactivation or protein aggregation. The role of proximal multi-phosphorylation events is becoming increasingly recognized as mechanistically important, although breakthroughs are limited due to a lack of detection technologies. To date, there is a limited selection of facile and robust sensing tools for proximal phosphorylation. Nonetheless, there have been considerable efforts in developing optical chemosensors for the detection of proximal phosphorylation motifs on peptides and proteins in recent years. This review provides a comprehensive overview of optical chemosensors for proximal phosphorylation, with the majority of work being reported in the past two decades. Optical sensors, in the form of fluorescent and luminescent chemosensors, hybrid biosensors, and inorganic nanoparticles, are described. Emphasis is placed on the rationale behind sensor scaffolds, relevant protein motifs, and applications in protein biology.

中文翻译：

用于检测近端磷酸化肽和蛋白质的光学化学传感器

近端多位点磷酸化是蛋白质生物学中关键的翻译后修饰。空间邻近的多个磷酸位点簇的累加效应引发对蛋白质构象和活性的综合和协同效应。近端磷酸化已被证明可以调节信号转导途径和基因表达，因此，通过改变蛋白质功能和/或定位（包括酶过度激活或蛋白质聚集）与多种疾病状态有关。尽管由于缺乏检测技术，突破受到限制，但近端多磷酸化事件的作用越来越被认为在机制上很重要。迄今为止，用于近端磷酸化的简便且强大的传感工具的选择有限。尽管如此，近年来，人们在开发用于检测肽和蛋白质上的近端磷酸化基序的光学化学传感器方面做出了相当大的努力。这篇综述对近端磷酸化光学化学传感器进行了全面的概述，其中大部分工作是在过去二十年中报道的。描述了荧光和发光化学传感器、混合生物传感器和无机纳米颗粒形式的光学传感器。重点放在传感器支架、相关蛋白质基序以及蛋白质生物学应用背后的基本原理。

更新日期：2021-04-29

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