Purpose

There is a growing need to use green and efficient larvicidal as alternatives for conventional chemicals in vector control programs. Nanotechnology has provided a promising approach for research and development of new larvicides. Larvicidal potential of a nanoemulsion of Cinnamomum zeylanicum essential oil reports against Anopheles stephensi.

Methods

The nanoemulsion of was formulated in various ratios comprising of C. zeylanicum oil, tween 80, span 20 and water by stirrer. It was characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). All components of C. zeylanicum essential oil were identified by GC–MS analysis. The larvicidal potential of the oil and its nanoformulation were evaluated against larvae of An. stephensi. The stability and durability of nanoemulsion was observed over a period of time.

Results

Sixty one components in the oil were identified, cinnamaldehyde (56.803%) was the main component. The LC₉₀ and LC₅₀ values of C. zeylanicum essential oil were calculated as 49 ppm and 37 ppm, respectively. The nanoemulsion droplets were found spherical in shape. It was able to kill 100% of larvae in up to 3 days. It was stable after dilution and increased its larvicidal activity up to 32% compared with the essential oil.

Conclusions

A novel larvicide based on nanotechnology introduced. This experiment clearly showed increasing larvicidal activity and residual effect of the nanoformulation in comparison with the bulk essential oil. It could be concluded that this nanoemulsion may be considered as safe larvicide and should be subject of more research in this field.

Graphical abstract

中文翻译：

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肉桂油纳米乳的制备及其对主要疟疾载体斯蒂芬斯按蚊的杀幼虫活性评价

目的

在病媒控制计划中，越来越需要使用绿色和高效的杀幼虫剂作为常规化学品的替代品。纳米技术为新型杀幼虫剂的研究和开发提供了一种很有前景的方法。肉桂精油纳米乳液的杀幼虫潜力对斯蒂芬按蚊的报道。

方法

通过搅拌器将纳米乳剂配制成不同的比例，包括C. zeylanicum油、吐温80、司盘20和水。通过透射电子显微镜 (TEM) 和动态光散射 (DLS) 对其进行了表征。通过 GC-MS 分析鉴定C. zeylanicum精油的所有成分。油及其纳米制剂的杀幼虫潜力针对An 的幼虫进行了评估。斯蒂芬西_ 在一段时间内观察纳米乳液的稳定性和耐久性。

结果

鉴定出油中61种成分，肉桂醛（56.803%）是主要成分。C. zeylanicum精油的 LC ₉₀和 LC ₅₀值分别计算为 49 ppm 和 37 ppm。发现纳米乳液液滴呈球形。它能够在 3 天内杀死 100% 的幼虫。稀释后稳定，与精油相比，杀幼虫活性提高32%。

结论

介绍了一种基于纳米技术的新型杀幼虫剂。该实验清楚地表明，与散装精油相比，纳米制剂的杀幼虫活性和残留效果增加。可以得出结论，这种纳米乳可以被认为是一种安全的杀幼虫剂，应该成为该领域更多研究的主题。

图形概要