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MiR-181a-5p inhibits goose granulosa cell viability by targeting SIRT1
British Poultry Science ( IF 1.6 ) Pub Date : 2021-01-25 , DOI: 10.1080/00071668.2020.1870660
Y. Rong 1 , Y. Mo 1 , Y. Liu 1 , Y. Deng 1 , S. Hu 1 , L. Li 1 , J. Hu 1 , B. Hu 1 , H. He 1 , J. Wang 1
Affiliation  

ABSTRACT

1. Granulosa cells (GCs) are involved in folliculogenesis, follicular development, and atresia. Previous studies have shown that microRNA-181a-5p (miR-181a-5p) and sirtuin 1 (SIRT1) are involved in GC proliferation and apoptosis, and SIRT1 has been predicted as one target of miR-181a-5p. However, there are few studies with poultry.

2. Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of miR-181a-5p in granulosa layers during geese ovarian follicular development. A methyl thiazolyl tetrazolium (MTT) assay was performed to assess the viability of geese granulosa cells treated with miR-181a-5p mimic or inhibitor. The binding sites between the SIRT1 3′-UTR region and miR-181a-5p were evaluated using a luciferase reporter assay system. SIRT1 mRNA levels were detected using qRT-PCR after transfection with miR-181a-5p mimic and inhibitor.

3. The miR-181a-5p suppressed geese GC viability and regulated the mRNA expression of viability-related genes in geese GCs. SIRT1 was a target gene of miR-181a-5p and miR-181a-5p suppressed its mRNA expression.

4. The miR-181a-5p may target and inhibit SIRT1 expression, thus suppressing GC viability by regulating viability-related key genes.



中文翻译:

MiR-181a-5p通过靶向SIRT1抑制鹅颗粒细胞的生存能力

摘要

1.颗粒细胞(GCs)参与卵泡形成,卵泡发育和闭锁。先前的研究表明microRNA-181a-5p(miR-181a-5p)和sirtuin 1(SIRT1)参与GC增殖和凋亡,并且SIRT1被预测为miR-181a-5p的靶标之一。但是,关于家禽的研究很少。

2.定量实时荧光定量PCR(qRT-PCR)用于检测鹅卵泡发育过程中颗粒层中miR-181a-5p的表达水平。进行了甲基噻唑基四唑鎓(MTT)分析,以评估用miR-181a-5p模拟物或抑制剂处理的鹅颗粒细胞的活力。使用萤光素酶报告基因测定系统评估SIRT1 3'-UTR区与miR-181a-5p之间的结合位点。用miR-181a-5p模拟物和抑制剂转染后,使用qRT-PCR检测SIRT1 mRNA水平。

3. miR-181a-5p抑制了鹅GC的生存能力,并调节了鹅GC中与生存能力相关的基因的mRNA表达。SIRT1是miR-181a-5p的靶基因,miR-181a-5p抑制了其mRNA表达。

4. miR-181a-5p可能靶向并抑制SIRT1表达,从而通过调节生存力相关关键基因来抑制GC生存力。

更新日期:2021-01-25
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