Histological, immunohistochemical, and molecular investigation on the hepatotoxic effect of potassium dichromate and the ameliorating role of Persea americana mill pulp extract,Microscopy Research and Technique

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Histological, immunohistochemical, and molecular investigation on the hepatotoxic effect of potassium dichromate and the ameliorating role of Persea americana mill pulp extract
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-04-27 , DOI: 10.1002/jemt.23798
Basma Emad Aboulhoda ₁ , Shimaa Saad El-Din ₂ , Mohamed Mansour Khalifa _{3,

4} , Sherif Fahmy Arsanyos ₁ , Ahmed Galal Motawie ₁ , Mohamed Shahat Sedeek ₅ , Gaber Hassan Abdelfattah ₆ , Waleed Ahmed Abdelgalil ₁

Affiliation

The current study has been designed to assess the role of Persea americana (P. americana) pulp extract on potassium dichromate-induced hepatotoxicity in rats. P. americana pulp extract administration improved the hepatic vascular congestion, blood extravasation, inflammatory cellular infiltration, Kupffer cell hyperplasia, and nuclear changes. It also significantly ameliorated hepatic interstitial and peri-portal fibrosis and caused retrieval of the PAS-positive reaction in the liver parenchyma and around the central vein with restoration of the glycogen granules. P. americana also significantly attenuated the immunohistochemical expression of NF-kβ p65 and its downstream inflammatory cytokines IL6 and TNFα in the liver parenchyma. The antioxidant effect of P. americana was evidenced by significant modulation of the three major components of the thioredoxin (Trx) antioxidant system, the Trx, the thioredoxin reductase (TrxR), and the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase along with significant increase in the level of superoxide dismutase and glutathione, and decrease in the lipid peroxidation product malondialdehyde. P. americana pulp extract also caused significant elevation of hepatic protein phosphatase 5 with subsequent down-regulation of Apoptosis signal-regulating kinase1 (ASK1) and its downstream signaling targets MAPK kinase 4 (MKK4), p38 mitogen-activated protein kinases (p38-MAPKs), the c-JUN N-terminal kinase (JNK), and the extracellular signal-regulated kinase 1/2 (ERK 1/2). Also, In conclusion, P. americana pulp extract has anti-oxidative and anti-inflammatory effects against potassium dichromate-induced hepatotoxicity.

中文翻译：

组织学、免疫组织化学和分子研究重铬酸钾的肝毒性作用和 Persea americana 纸浆提取物的改善作用

目前的研究旨在评估Persea americana ( P. americana ) 果肉提取物对重铬酸钾诱导的大鼠肝毒性的作用。美洲松果肉提取物给药改善了肝血管充血、血液外渗、炎性细胞浸润、枯否细胞增生和核变化。它还显着改善了肝间质和门静脉周围纤维化，并通过恢复糖原颗粒使肝实质和中央静脉周围的 PAS 阳性反应恢复。美洲山雀还显着减弱了肝实质中 NF-kβ p65 及其下游炎性细胞因子 IL6 和 TNFα 的免疫组织化学表达。P. americana的抗氧化作用通过硫氧还蛋白 (Trx) 抗氧化系统的三个主要成分 Trx、硫氧还蛋白还原酶 (TrxR) 和烟酰胺腺嘌呤二核苷酸磷酸 (NADPH) 氧化酶的显着调节以及显着增加来证明超氧化物歧化酶和谷胱甘肽的水平，以及脂质过氧化产物丙二醛的降低。美洲山雀果肉提取物还导致肝蛋白磷酸酶 5 显着升高，随后下调凋亡信号调节激酶 1 (ASK1) 及其下游信号靶标 MAPK 激酶 4 (MKK4)、p38 丝裂原活化蛋白激酶 (p38-MAPK)、 c-JUN N 端激酶 (JNK) 和细胞外信号调节激酶 1/2 (ERK 1/2)。此外，总而言之，美洲松果肉提取物对重铬酸钾引起的肝毒性具有抗氧化和抗炎作用。

更新日期：2021-04-27

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