In this study, it was aimed to reveal the potential of using exopolysaccharides (EPS) obtained from Ligilactobacillus salivarius as a prebiotic that regulates chicken intestinal microbiota. Characterization of EPS obtained from L. salivarius BIS312 (EPSBIS312) and BIS722 (EPSBIS722) strains was demonstrated by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and size-exclusion chromatography (SEC) analyses. It was determined that the molecular weight of both EPS is in the range of 10⁴–10⁶ Daltons, and there are 4 types of monomers in their structure. Anti-biofilm and anti-quorum sensing effects of EPSBIS312 and EPSBIS722 were determined. EPSBIS312 and EPSBIS722 showed a strong anti-biofilm effect on Enterococcus faecalis ATCC 29212, Staphylococcus aureus EB-1, and Escherichia coli ATCC 11229. The anti-quorum sensing study revealed that the EPSBIS722 had a higher effect than the EPSBIS312. The effect of different concentrations of EPS (2.5%, 5%, 10%) on lactobacilli growth stimulator (LGS) was evaluated. The highest LGS was promoted at 10% concentration while the lowest LGS was promoted at 2.5% concentration by EPSBIS722. In addition, adhesion abilities of EPSBIS312 and EPSBIS722 in HT-29 colorectal adenocarcinoma cell line were tested. EPSs significantly increased the ability to adhere to HT-29 cells. The characterized EPSs may be an alternative to plant prebiotics such as inulin at poultry.

在这项研究中，旨在揭示使用从唾液乳杆菌中获得的胞外多糖 (EPS)作为调节鸡肠道微生物群的益生元的潜力。高效液相色谱 (HPLC)、核磁共振 (NMR) 和尺寸排阻色谱 (SEC) 分析证明了从L. salivarius BIS312 (EPSBIS312) 和 BIS722 (EPSBIS722) 菌株获得的 EPS 的表征。经测定，两种EPS的分子量都在10 ⁴ -10 ⁶道尔顿范围内，其结构中有4种单体。测定了 EPSBIS312 和 EPSBIS722 的抗生物膜和抗群体感应作用。EPSBIS312 和 EPSBIS722 对粪肠球菌ATCC 29212、金黄色葡萄球菌EB-1 和大肠杆菌ATCC 11229。抗群体感应研究表明，EPSBIS722 的效果高于 EPSBIS312。评价了不同浓度EPS（2.5%、5%、10%）对乳酸杆菌生长刺激剂（LGS）的影响。EPSBIS722 在 10% 浓度时促进了最高 LGS，而在 2.5% 浓度时促进了最低 LGS。此外，测试了EPSBIS312和EPSBIS722在HT-29结直肠腺癌细胞系中的粘附能力。EPS显着增加了粘附HT-29细胞的能力。表征的 EPS 可以替代植物益生元，例如家禽中的菊粉。