Mitochondria play a critical role in cell function and embryo development. Recently, increasing studies have investigated whether mitochondrial DNA (mtDNA) can be used as a predictive biomarker of embryo implantation. However, the results of its effect on implantation are still controversial. To further understand the clinical application value of mtDNA content for reproductive potential, we analyzed the influence of relative mtDNA quantity on embryo quality and transfer outcomes based on the results of second-generation sequencing of preimplantation genetic testing patients in our center. Biopsied trophectoderm (TE) from aneuploid blastocysts contained much larger amounts of mtDNA than those from euploid blastocysts (p < 0.000). In an analysis of only euploid blastocysts (n = 769), female age had no effect on mtDNA content (p = 0.216). TE cells biopsied on day 5 (n = 355) contained significantly higher amounts of mtDNA compared to those biopsied on day 6 (n = 388) or day 7 (n = 26) (p < 0.000). Higher quality trophoblast was associated with lower mtDNA content (p = 0.026), but quality of inner cell mass was not correlated with quantity of mtDNA (p = 0.112). For transferred embryos, the biopsied date and mtDNA content were significantly associated with embryo implantation and live birth outcomes. Day-5 euploid blastocysts with lower quantities of mtDNA exhibited higher implantation rate and live birth rate. However, our data indicated that mtDNA content may not be considered an independent predictive marker, it may be a useful reference for the selection of day-5 transferred euploid blastocysts.

中文翻译：

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滋养外胚层线粒体 DNA 含量与胚胎质量和第 5 天整倍体囊胚移植结果相关


线粒体在细胞功能和胚胎发育中发挥着关键作用。最近，越来越多的研究调查线粒体 DNA (mtDNA) 是否可以用作胚胎植入的预测生物标志物。然而，其对植入的影响结果仍存在争议。为了进一步了解mtDNA含量对生殖潜能的临床应用价值，我们根据本中心植入前基因检测患者的二代测序结果，分析了mtDNA相对含量对胚胎质量和移植结局的影响。来自非整倍体囊胚的活检滋养外胚层 (TE) 比来自整倍体囊胚的滋养外胚层 (TE) 含有更多量的 mtDNA ( p < 0.000)。在仅对整倍体囊胚进行的分析中 ( n = 769)，女性年龄对 mtDNA 含量没有影响 ( p = 0.216)。与第 6 天 ( n = 388) 或第 7 天 ( n = 26) 活检的细胞相比，第 5 天 ( n = 355) 活检的 TE 细胞含有明显更高量的 mtDNA ( p < 0.000)。较高质量的滋养层与较低的 mtDNA 含量相关（ p = 0.026），但内细胞团的质量与 mtDNA 的数量无关（ p = 0.112）。对于移植胚胎，活检日期和线粒体 DNA 含量与胚胎植入和活产结果显着相关。线粒体 DNA 含量较低的第 5 天整倍体囊胚表现出较高的着床率和活产率。然而，我们的数据表明mtDNA含量可能不被认为是一个独立的预测标记，它可能对第5天转移的整倍体囊胚的选择有一个有用的参考。