ABSTRACT

The aim of this study was to synthesize chitosan nanoparticles loaded with rapeseed extract (RPE-CN) and to evaluate its therapeutic effects. The RPE-CNwas synthesized (Ion gelation), characterized (DLS, FTIR, atomic and electron microscopy) and their toxicity was evaluated by MTT method.Anti-inflammatory and pro-apoptotic effects were evaluated by qPCR method. Its antioxidant power was measured by ABTS, DPPH and FRAP methods and CAM method was used to evaluate its anti-angiogenic effects.Treatment with RPE-CN(Ps: 93.81 nm, PDI: 0.3, and ζ p: +35mv) reduced MCF7 cell viability and increased expression of genes associated with apoptosis (Caspase-3 and Caspase-9), inflammation (IL10) and antioxidant (SOD).RPE-CN exhibited notable inhibition in ABTS, DPPH, and Fe3+-TPTZ free radicals. Finally, angiogenesis in CAM tissue was suppressed by the down-regulation of VEGFR gene expression. According to the results, RPE-CN can be introduced as a potential chemo-preventive agent in the treatment of cancer.

摘要

本研究的目的是合成负载油菜籽提取物 (RPE-CN) 的壳聚糖纳米颗粒并评估其治疗效果。合成RPE-CN（离子凝胶），表征（DLS，FTIR，原子和电子显微镜）并通过MTT方法评估其毒性。通过qPCR方法评估抗炎和促凋亡作用。用ABTS、DPPH和FRAP法测定其抗氧化能力，用CAM法评价其抗血管生成作用。用RPE-CN(Ps: 93.81 nm, PDI: 0.3, and ζ p: +35mv)处理减少MCF7细胞与细胞凋亡（Caspase-3 和 Caspase-9）、炎症（IL10）和抗氧化剂（SOD）相关的基因的活力和表达增加。RPE-CN 在 ABTS、DPPH 和 Fe3+-TPTZ 自由基中表现出显着的抑制作用。最后，VEGFR基因表达的下调抑制了CAM组织中的血管生成。根据结果​​，RPE-CN可以作为一种潜在的化学预防剂用于癌症的治疗。