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Development of HPTLC detection of synthetic pesticide carbosulfan in biological material
JPC - Journal of Planar Chromatography - Modern TLC ( IF 1.1 ) Pub Date : 2021-04-27 , DOI: 10.1007/s00764-021-00096-7
Umakant D. Pawar , Chandrakant D. Pawar , Dattatraya N. Pansare , Jayanti G. Humbe , Rajendra K. Pardeshi

The field of agriculture is the key role in Indian society in which insecticides play a vital role in increasing the crop production yield. Carbosulfan is a member of the carbamate family; it is an important insecticide and it has a diversified role in agriculture. There is an increasing number of human poisoning cases by mainly the compound carbosulfan of the carbamate family. During the last few years, Forensic Science Laboratories of Maharashtra, India, detected a large number of human poisoning cases with carbosulfan since a large number of biological samples (postmortem sample) were received for toxicological chemical analysis. Thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) were the preferred methods of choice. By observing the results of toxicological chemical analysis, the conclusion can be drawn that carbosulfan plays a major role in poisoning cases. The chromogenic reagent was found to be a selective spray reagent for carbosulfan in routine analysis by HPTLC. Carbosulfan reacts with chromogenic reagent which gives more intense pink-colored compounds than the other organochlorine insecticides, organophosphorus insecticides, and pyrethroid insecticides. Biological material constituents (amino acids, peptides, proteins, fats, etc.) do not interfere with this chromogenic reagent.



中文翻译:

生物材料中合成农药硫丹的HPTLC检测方法的建立

农业领域是印度社会的关键角色,在这种社会中,杀虫剂在提高农作物产量中起着至关重要的作用。卡巴硫丹是氨基甲酸酯家族的成员。它是一种重要的杀虫剂,在农业中具有多种作用。主要由氨基甲酸酯家族的化合物复方硫丹引起的人中毒案件数量在增加。在最近几年中,印度马哈拉施特拉邦法医学实验室发现了大量的人因硫丹而引起中毒,因为已收到大量生物样品(尸体样品)用于毒理学化学分析。首选的方法是薄层色谱(TLC)和高性能薄层色谱(HPTLC)。通过观察毒理化学分析的结果,可以得出结论,硫丹在中毒案件中起主要作用。在HPTLC的常规分析中,发色剂被发现是对硫丹的选择性喷雾剂。卡硫丹与生色剂反应,与其他有机氯杀虫剂,有机磷杀虫剂和拟除虫菊酯类杀虫剂相比,产生更强烈的粉红色化合物。生物材料成分(氨基酸,肽,蛋白质,脂肪等)不会干扰该生色试剂。和拟除虫菊酯类杀虫剂。生物材料成分(氨基酸,肽,蛋白质,脂肪等)不会干扰该生色试剂。和拟除虫菊酯类杀虫剂。生物材料成分(氨基酸,肽,蛋白质,脂肪等)不会干扰该生色试剂。

更新日期:2021-04-28
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