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A Genomic Analysis of Historic Chickpea Landraces
Biophysics Pub Date : 2021-04-27 , DOI: 10.1134/s0006350921010061
A. B. Sokolkova , S. V. Bulyntsev , P. L. Chang , N. Carrasquila-Garcia , D. R. Cook , E. von Wettberg , M. A. Vishnyakova , S. V. Nuzhdin , M. G. Samsonova

The collection of seeds and living plants at the N.I. Vavilov All Russian Institute of Plant Genetic Resources (VIR) contains historic chickpea landraces sampled from major historic centers of chickpea cultivation and secondary diversification. In this work, we analyze the results obtained from phenotype characterization of 407 historic chickpea landraces grown in the trial fields of Kuban experimental station in 2017. GWAS analysis identified three single nucleotide polymorphisms on chromosomes 2, 7 and 8 that were highly associated with the days to flowering phenotype trait. These single nucleotide polymorphisms and the regions of genome near to these single nucleotide polymorphisms were identified in previous studies. Estimation of the Genotype–Environment interaction for phenotypic traits coincident with plant seeds led to identification of the best genotypes in the environments. These genotypes have an alternative homozygote at loci Ca7:30930779 which shows similar phenotypic traits that were described during phenotype characterization of seeds grown in the trial fields of the Kuban experimental station in 2016 and with the period of flowering typical of phenotype characterization of the plants in 2017. These results can accelerate the search for the seed samples that are the most promising for cultivation of chickpea landraces.



中文翻译:

历史悠久的鹰嘴豆地方品种的基因组分析

NI Vavilov全俄罗斯植物遗传资源研究所(VIR)收集的种子和有生命的植物包含从鹰嘴豆栽培和次生多元化的主要历史中心取样的历史性鹰嘴豆地方品种。在这项工作中,我们分析了2017年在库班实验站试验田中生长的407种历史性鹰嘴豆地方品种的表型表征结果。GWAS分析确定了与天数高度相关的3号染色体上的三个单核苷酸多态性表现出开花表型性状。这些单核苷酸多态性和靠近这些单核苷酸多态性的基因组区域在先前的研究中已经确定。表型性状与植物种子的基因型-环境相互作用的估计导致鉴定环境中最佳的基因型。这些基因型在Ca7:30930779位点具有另一种纯合子,表现出相似的表型特征,该特征在2016年Kuban实验站试验田中生长的种子的表型表征期间描述,并且在开花期表现出典型的植物表型表征。 2017年。这些结果可以加快对鹰嘴豆地方品种的种植最有希望的种子样品的搜索。

更新日期:2021-04-28
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