It is shown that the inhalation of gaseous nitric oxide (gNO) or sprayed aqueous solutions of binuclear dinitrosyl iron complexes with glutathione or N-acetyl-L-cysteine by animals or humans provokes no perceptible hypotensive effects. Potentially, these procedures may be useful in COVID-19 treatment. The NO level in complexes with hemoglobin in blood decreases as the gNO concentration in the gas flow produced by the Plazon system increases from 100 to 2100 ppm, so that at 2000 ppm more than one-half of the gas can be incorporated into dinitrosyl complexes formed in tissues of the lungs and respiratory tract. Thus, the effect of gNO inhalation may be similar to that observed after administration of solutions of dinitrosyl iron complexes, namely, to the presence of dinitrosyl iron complexes with thiol-containing ligands in lung and airway tissues. With regard to the hypothesis posited earlier that these complexes can suppress coronavirus replication as donors of nitrosonium cations (Biophysics 65, 818, 2020), it is not inconceivable that administration of gNO or chemically synthesized dinitrosyl iron complexes with thiol-containing ligands may help treat COVID-19. In tests on the authors of this paper as volunteers, the tolerance concentration of gNO inhaled within 15 min was approximately 2000 ppm. In tests on rats that inhaled sprayed aqueous solutions of dinitrosyl iron complexes, their tolerance dose was approximately 0.4 mmol/kg body weight.

研究表明，动物或人类吸入气态一氧化氮（gNO）或喷洒双核二亚硝酰铁与谷胱甘肽或N-乙酰基-L-半胱氨酸复合物的水溶液不会引起明显的降血压作用。这些程序可能对 COVID-19 治疗有用。随着 Plazon 系统产生的气流中 gNO 浓度从 100 增加到 2100 ppm，血液中与血红蛋白形成的复合物中的 NO 水平降低，因此在 2000 ppm 时，超过一半的气体可以结合到形成的二亚硝基复合物中在肺和呼吸道组织中。因此，吸入gNO的效果可能与施用二亚硝酰铁络合物溶液后观察到的效果相似，即与肺和气道组织中存在含硫醇配体的二亚硝酰铁络合物相似。关于之前提出的假设，即这些复合物可以作为亚硝鎓阳离子供体抑制冠状病毒的复制（生物物理学 65, 818, 2020），给予 gNO 或化学合成的二亚硝基铁复合物与含硫醇配体可能有助于治疗冠状病毒并非不可想象。 2019冠状病毒病。本文作者作为志愿者进行的测试中，15分钟内吸入的gNO耐受浓度约为2000 ppm。在对吸入二亚硝基铁络合物喷雾水溶液的大鼠进行的试验中，其耐受剂量约为0.4mmol/kg体重。