There is a growing concern regarding the adverse effects of pharmaceutical pollution on aquatic environments. The present study investigated the toxicity of different antibiotics and non-steroidal anti-inflammatory drugs (NSAIDs) on two green algae namely Chlorella sp. and Desmodesmus spinosus. Based on the 96h IC₅₀ values of the two chlorophytes, tetracycline (TET) was more toxic than other antibiotics (ciprofloxacin (CPF) and amoxicillin (AMX)), while paracetamol (PAR) was more toxic than ketoprofen (KET) and diclofenac (DIF). Gross photosynthesis was markedly reduced with most of the investigated drugs, although, the Chl a content was stimulated in a dose-dependent manner. Algal treated cells exhibited elevated malonaldehyde content which reflected several structural and functional cellular damages. Catalase and ascorbate peroxidase (APX) were involved in the reduction of reactive oxygen species, but the effects of APX were more pronounced at low drug concentrations. Box–Behnken design (BBD) was employed to investigate the combined toxicities of PAR, KET, AMX and TET on D. spinosus in response to growth inhibition and pigment increase. The mutual interactions varied between synergism and antagonism. The BBD analysis indicated that the experimental design could be effectively utilized as a useful tool for ecotoxicological assessment.

人们越来越关注药物污染对水生环境的不利影响。本研究调查了不同的抗生素和非甾体抗炎药（NSAIDs）对两种绿藻，即小球藻（Chlorella sp ）的毒性。和Desmodesmus spinosus。基于96h IC ₅₀两种叶绿素的四环素（TET）值比其他抗生素（环丙沙星（CPF）和阿莫西林（AMX））更具毒性，而对乙酰氨基酚（PAR）的毒性大于酮洛芬（KET）和双氯芬酸（DIF）。尽管大多数被研究药物的总光合作用均明显降低，但其Chla含量却以剂量依赖的方式被刺激。经藻处理的细胞显示出较高的丙二醛含量，这反映了几种结构和功能性细胞损伤。过氧化氢酶和抗坏血酸过氧化物酶（APX）参与了活性氧的减少，但在低药物浓度下，APX的作用更为明显。采用Box–Behnken设计（BBD）来研究PAR，KET，AMX和TET对棘球藻的综合毒性响应生长抑制和色素增加。协同作用和拮抗作用之间的相互作用互不相同。BBD分析表明，实验设计可以有效地用作生态毒理学评估的有用工具。