During salmonid spawning, the amount of time required for activated milt to successfully fertilize eggs has not been well defined. This study examined the effects of two different activated milt residence times, 20 and 60 s, during production-scale artificial spawning of 20 landlocked fall Chinook Salmon Oncorhynchus tshawytscha. Mean (SE) survival to the eyed stage of egg development was 29.2% (5.5) in the eggs that were subjected to the 20-s milt residence time, which was not significantly different from the 32.3% (5.7) survival rate for eggs with the 60-s residence time. There was considerable variation in egg survival among the individual female spawns, ranging from near 0% to 76.7%. These production-scale results indicate that activated milt residence times as low as 20 s can be used during the landlocked fall Chinook Salmon spawning, with no significant effect on egg survival to the eyed stage of development.

中文翻译：

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两个活化的鱼苗停留时间对内陆秋季奇努克鲑鱼卵存活至眼发育阶段的影响

在鲑鱼产卵期间，活化的鱼精成功使卵受精所需的时间尚未明确。本研究检查了两种不同的活化鱼糜停留时间（20 秒和 60 秒）在 20 只内陆秋季奇努克三文鱼Oncorhynchus tshawytscha 的生产规模人工产卵过程中的影响. 在经历 20 秒 milt 停留时间的鸡蛋中，鸡蛋发育的眼睛阶段的平均 (SE) 存活率为 29.2% (5.5)，这与 32.3% (5.7) 的鸡蛋存活率没有显着差异。 60 秒的停留时间。个体雌性产卵的卵存活率存在相当大的差异，从接近 0% 到 76.7% 不等。这些生产规模的结果表明，在内陆秋季奇努克鲑鱼产卵期间可以使用低至 20 秒的活化鱼白滞留时间，对卵存活到眼睛发育阶段没有显着影响。