当前位置：
X-MOL 学术
›
J. Neuroendocrinol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Hormonal and neural responses to restraint stress in an animal model of perimenopause in female rats
Journal of Neuroendocrinology ( IF 3.3 ) Pub Date : 2021-04-26 , DOI: 10.1111/jne.12976 Adesina Paul Arikawe 1, 2 , Rodrigo César Rorato 3, 4 , Nathali Gomes 3 , Lucila Leico Elias 3 , Janete Anselmo-Franci 1
Journal of Neuroendocrinology ( IF 3.3 ) Pub Date : 2021-04-26 , DOI: 10.1111/jne.12976 Adesina Paul Arikawe 1, 2 , Rodrigo César Rorato 3, 4 , Nathali Gomes 3 , Lucila Leico Elias 3 , Janete Anselmo-Franci 1
Affiliation
|
The present study investigated the hormonal and neural responses to stress in a perimenopause animal model induced by 4-vinylcyclohexene diepoxide (VCD), which induces progressive follicular depletion in rodents, allowing studies on the transition to ovarian failure. Female rats, aged 28 days old, were s.c. injected for 15 consecutive days with corn oil or VCD. At 85 ± 5 days after the onset of treatment, the jugular vein was cannulated in the afternoon of metoestrus and in next morning (dioestrus) at 10.00 am, rats were subjected to 30 minutes of restraint stress. Blood samples were withdrawn before (-5 minutes), during (2, 5, 15 and 30 minutes) and after (45, 60 and 90 minutes) stress and plasma prolactin, progesterone and corticosterone levels were measured. Animals were perfused, brains processed for c-Fos/tyrosine hydroxylase (TH) in the locus coeruleus (LC) and c-Fos/corticotrophin-releasing factor (CRF) in the paraventricular nucleus (PVN). In unstressed rats the density of β-endorphin fibres was assessed in LC and PVN. In VCD-treated rats, stress-induced prolactin peak was higher, basal and peak progesterone levels were lower, and both levels of corticosterone were similar to controls. However, the recovery period was longer for both adrenal hormones. In VCD-treated rats the number of c-Fos/TH and c-Fos/CRF-immunoreactive neurones was higher whereas the density of β-endorphin fibres was lower in LC and PVN. We surmise that the hyperactivity of the LC and PVN neurones in VCD-treated rats may be a result of the lower progesterone levels that resulted in the decrease of β-endorphin content in both nuclei, thus impairing the negative-feedback mechanism in the recovery period.
中文翻译：
雌性大鼠围绝经期动物模型对束缚应激的激素和神经反应
本研究调查了 4-乙烯基环己烯二环氧化物 (VCD) 诱导的围绝经期动物模型对压力的激素和神经反应，VCD 会诱导啮齿类动物进行性卵泡耗竭，从而可以研究卵巢功能衰竭的转变。 28日龄雌性大鼠连续15天皮下注射玉米油或VCD。治疗开始后85±5天，在发情后期下午和第二天早上（动情期）上午10点对大鼠进行颈静脉插管，使大鼠受到30分钟的束缚应激。在应激之前（-5分钟）、期间（2、5、15和30分钟）和之后（45、60和90分钟）抽取血样，并测量血浆催乳素、孕酮和皮质酮水平。对动物进行灌注，对蓝斑（LC）中的c-Fos/酪氨酸羟化酶（TH）和室旁核（PVN）中的c-Fos/促肾上腺皮质激素释放因子（CRF）进行大脑处理。在未受应激的大鼠中，在 LC 和 PVN 中评估 β-内啡肽纤维的密度。在 VCD 治疗的大鼠中，应激诱导的催乳素峰值较高，基础孕酮水平和峰值孕酮水平较低，皮质酮水平与对照组相似。然而，两种肾上腺激素的恢复期都较长。在VCD处理的大鼠中，LC和PVN中c-Fos/TH和c-Fos/CRF免疫反应性神经元的数量较高，而β-内啡肽纤维的密度较低。我们推测VCD治疗大鼠LC和PVN神经元的过度活跃可能是由于黄体酮水平较低导致两个细胞核中β-内啡肽含量减少，从而损害恢复期的负反馈机制的结果。
更新日期：2021-05-25
中文翻译：
雌性大鼠围绝经期动物模型对束缚应激的激素和神经反应
本研究调查了 4-乙烯基环己烯二环氧化物 (VCD) 诱导的围绝经期动物模型对压力的激素和神经反应，VCD 会诱导啮齿类动物进行性卵泡耗竭，从而可以研究卵巢功能衰竭的转变。 28日龄雌性大鼠连续15天皮下注射玉米油或VCD。治疗开始后85±5天，在发情后期下午和第二天早上（动情期）上午10点对大鼠进行颈静脉插管，使大鼠受到30分钟的束缚应激。在应激之前（-5分钟）、期间（2、5、15和30分钟）和之后（45、60和90分钟）抽取血样，并测量血浆催乳素、孕酮和皮质酮水平。对动物进行灌注，对蓝斑（LC）中的c-Fos/酪氨酸羟化酶（TH）和室旁核（PVN）中的c-Fos/促肾上腺皮质激素释放因子（CRF）进行大脑处理。在未受应激的大鼠中，在 LC 和 PVN 中评估 β-内啡肽纤维的密度。在 VCD 治疗的大鼠中，应激诱导的催乳素峰值较高，基础孕酮水平和峰值孕酮水平较低，皮质酮水平与对照组相似。然而，两种肾上腺激素的恢复期都较长。在VCD处理的大鼠中，LC和PVN中c-Fos/TH和c-Fos/CRF免疫反应性神经元的数量较高，而β-内啡肽纤维的密度较低。我们推测VCD治疗大鼠LC和PVN神经元的过度活跃可能是由于黄体酮水平较低导致两个细胞核中β-内啡肽含量减少，从而损害恢复期的负反馈机制的结果。
京公网安备 11010802027423号