This study investigated the potential functions of Pleurotus florida (an edible mushroom) in the biodegradation of gas oil at concentrations of 0 (control), 2.5, 5, and 10% (V: V) for 30 days. The gas oil increased dry weight and protein concentration in all treatments (by an average of 19.5 and 108%, respectively). Moreover, the pH, surface tension (ST), and interfacial tension (IFT) were reduced by the mushroom supplementation. The lowest surface tension (31.9 mN m⁻¹) and the highest biosurfactant production belonged to the 10% gas oil treatment (0.845 ± 0.03 mg mL⁻¹). The results demonstrated that the adsorption isotherm agreed well with the Langmuir isotherm. The maximum Langmuir adsorption capacity was calculated at 0.743 mg g⁻¹ wet biomass of P. florida. The fungal supplementation efficiently remedied the total petroleum hydrocarbons (TPHs) by an average of 55% after 30 days. Gas chromatography (GC) analysis revealed that P. florida effectively detoxified C₁₃–C₂₈ hydrocarbons, Pristane, and Phytane, implying its high mycoremediation function. The toxicity test showed that mycoremediation increased the germination by an average of 35.82% ± 8.89 after 30 days. Laccase activity increased significantly with increasing gas oil concentration in the treatments. The maximum laccase activity was obtained in the 10% gas oil treatment (142.25 ± 0.72 U L⁻¹). The presence of pollutants was also associated with induction in the tyrosinase activity when compared to the control. These results underline the high mycoremediation capacity of P. florida through the involvement of biosurfactants, laccase, and tyrosinase.

本研究调查了佛罗里达平菇（一种食用蘑菇）在 0（对照）、2.5、5 和 10% (V: V) 浓度下生物降解瓦斯油 30 天的潜在功能。在所有处理中，瓦斯油增加了干重和蛋白质浓度（分别平均增加了 19.5% 和 108%）。此外，添加蘑菇还降低了 pH、表面张力 (ST) 和界面张力 (IFT)。最低的表面张力 (31.9 mN m ^-1 ) 和最高的生物表面活性剂产量属于 10% 瓦斯油处理 (0.845 ± 0.03 mg mL ^-1 )。结果表明吸附等温线与朗缪尔等温线非常吻合。最大朗缪尔吸附容量计算为 0.743 mg g ^-1P. florida 的湿生物量。真菌补充剂在 30 天后有效地修复了总石油烃 (TPH) 平均 55%。气相色谱 (GC) 分析显示P. florida有效地解毒 C ₁₃ -C ₂₈碳氢化合物、Pristane 和植烷，暗示其具有高真菌介导功能。毒性试验表明，真菌介导在 30 天后使发芽率平均提高了 35.82%±8.89。漆酶活性随着处理中粗柴油浓度的增加而显着增加。在 10% 瓦斯油处理中获得最大漆酶活性 (142.25 ± 0.72 U L ^-1）。与对照相比，污染物的存在也与酪氨酸酶活性的诱导有关。这些结果强调了通过生物表面活性剂、漆酶和酪氨酸酶的参与，佛罗里达州假单胞菌的高真菌介导能力。