In this study, seven different cyanobacteria (LS01-LS07) were isolated from paddy field water and among them, the isolate LS04 was able to grow well on municipal wastewater. The LS04 isolate was identified as Nostoc sp. (designated as Nostoc sp. LS04) based on 16S rRNA gene sequence analysis. Strain LS04 grew well in 75% wastewater and had the greatest nutrients removal efficiency (81.02–95.17%). Strain LS04 obtained the higher biomass (1.31 ± 0.08 g L⁻¹) and productivity of 131.33 ± 8.08 mg L⁻¹ d⁻¹. The lipid content and productivity of LS04 were 14.85 ± 0.86% (dry cell weight) and 19.46 ± 0.05 mg L⁻¹ d⁻¹, respectively. The high proportion of C16–C18 fatty acids found in the lipids of LS04 indicated the high suitability for biodiesel production. In addition, Nostoc sp. LS04 cellular extracts were potentially used as a biostimulant for Lactuca sativa cultivation. The foliar application of 60% LS04 cellular extracts showed the maximum shoot length, root length, fresh biomass, dry biomass, Chl a, Chl b and carotenoids in lettuce plants compared to control plants. Similarly, 60% of LS04 cellular extracts treatment improved the concentrations of macro and micronutrients, and biochemical compounds in the leaves. Therefore, these results reveal that the Nostoc sp. LS04 is a promising candidate for the nutrients removal from wastewater and their biomass is a potential resource for biodiesel production and biostimulant for sustainable crop production.

在这项研究中，从稻田水中分离出了7种不同的蓝细菌（LS01-LS07），其中，分离株LS04能够在城市废水中生长良好。LS04分离株被鉴定为Nostoc sp。（命名为Nostoc sp.LS04）基于16S rRNA基因序列分析。LS04菌株在75％的废水中生长良好，并且具有最大的养分去除效率（81.02–95.17％）。菌株LS04获得更高的生物量（1.31±0.08g L ^-1）和131.33±8.08mg L ^-1 d ^-1的生产率。LS04的脂质含量和生产率为14.85±0.86％（干细胞重量）和19.46±0.05 mg L ^-1 d ^-1， 分别。在LS04的脂质中发现的高比例的C16–C18脂肪酸表明高度适合生物柴油的生产。此外，Nostoc sp。LS04细胞提取物潜在地用作用于生物刺激莴栽培。与对照植物相比，叶面施用60％LS04细胞提取物显示出莴苣植物的最大芽长，根长，新鲜生物量，干燥生物量，Chl a，Chl b和类胡萝卜素。同样，60％的LS04细胞提取物处理可改善叶片中大量和微量营养素以及生化化合物的浓度。因此，这些结果表明Nostocsp。LS04是从废水中去除营养物的有前途的候选者，其生物质是生物柴油生产和可持续作物生产的生物刺激物的潜在资源。