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Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity
Comparative Cytogenetics ( IF 1 ) Pub Date : 2021-04-26 , DOI: 10.3897/compcytogen.v15i2.60649
Pun Yeesin , Phichaya Buasriyot , Sukhonthip Ditcharoen , Patcharaporn Chaiyasan , Chatmongkon Suwannapoom , Sippakorn Juntaree , Sitthisak Jantarat , Sucheela Talumphai , Marcelo de Bello Cioffi , Thomas Liehr , Alongklod Tanomtong , Weerayuth Supiwong

Karyotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization (FISH) techniques. Microsatellite d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeat probes were applied in FISH. The obtained data revealed that the four studied species have different chromosome complements. The diploid chromosome numbers (2n) and the fundamental numbers (NF) range between 52 and 102, 54 and 104, 56 and 98, or 58 and 108 in M. mysticetus, M. atrifasciatus, M. singaringan or M. wolffii, respectively. Karyotype formulae of M. mysticetus, M. atrifasciatus, M. singaringan and M. wolffii are 24m+26sm+4a, 26m+24sm+2a, 24m+18sm+14a and 30m+22sm+6a, respectively. A single pair of NORs was identified adjacent to the telomeres of the short arm of chromosome pairs 3 (metacentric) in M. atrifasciatus, 20 (submetacentric) in M. mysticetus, 15 (submetacentric) in M. singaringan, and 5 (metacentric) in M. wolffii. The d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeats were abundantly distributed in species-specific patterns. Overall, we present a comparison of cytogenetic and molecular cytogenetic patterns of four species from genus Mystus providing insights into their karyotype diversity in the genus.

中文翻译:

来自泰国的四种Mystus物种(Bagridae,Siluriformes)的比较研究:对它们的核型多样性的见解

分析了Mystus Scopoli,1777(Bagridae),M。atrifasciatus Fowler,1937,M。mysticetus Roberts,1992,M。singaringan(Bleeker,1846)和M. wolffii(Bleeker,1851)的四种cat鱼的核型。通过常规和Ag-NOR条带以及荧光原位杂交(FISH)技术。在FISH中使用微卫星d(GC)15,d(CAA)10,d(CAT)10和d(GAA)10重复探针。获得的数据表明,这四个研究物种具有不同的染色体互补。在Mysticetus,M。atrifasciatus,M。singaringan或M. wolffii中,二倍体染色体数(2n)和基数(NF)分别在52和102、54和104、56和98或58和108之间。 。Mysticetus,M。atrifasciatus,M。singaringan和M.wolffii的核型公式为24m + 26sm + 4a,26m + 24sm + 2a,24m + 18sm + 14a和30m + 22sm + 6a。鉴定出一对NOR,与atrifasciatus中的染色体对3(超中心),mysticetus中的20(亚超中心),s.gargaringan中的15(亚超中心)和5(metacentric)染色体对的短臂端粒相邻。在沃尔夫菲(M. Wolffii)。d(GC)15,d(CAA)10,d(CAT)10和d(GAA)10重复序列以物种特异性模式大量分布。总体而言,我们比较了Mystus属中四种物种的细胞遗传学和分子细胞遗传学模式,提供了它们在该属中的核型多样性的见解。d(GC)15,d(CAA)10,d(CAT)10和d(GAA)10重复序列以物种特异性模式大量分布。总体而言,我们比较了Mystus属中四种物种的细胞遗传学和分子细胞遗传学模式,提供了它们在该属中的核型多样性的见解。d(GC)15,d(CAA)10,d(CAT)10和d(GAA)10重复序列以物种特异性模式大量分布。总体而言,我们比较了Mystus属中四种物种的细胞遗传学和分子细胞遗传学模式,提供了它们在该属中的核型多样性的见解。
更新日期:2021-04-27
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