Abstract

The effect of phenol (Ph, 0.25 mmol/L) on the activity and temperature dependence of casein- and hemoglobin-lytic peptidases functioning in the whole organism of chironomid larvae Chironomus sp. within the temperature range of 0−70°С at different рН values (3.0, 5.0, and 7.4) is studied. The temperature optimum of the studied peptidases of chironomid larvae corresponds to 40°С. Under the influence of phenol, the activity of casein-lytic peptidases increases more significantly at 20°С and рН 5.0 (by a factor of 3.3) and that of hemoglobin-lytic peptidases increases more significantly at рН 3.0 (by a factor of 3.5). The effect successively decreases from рН 3.0 up to рН 7.4. under both temperatures. In the presence of phenol, the shape of the curves of the temperature dependence and the Q₁₀ values transform most significantly in the zone of high temperatures. The values of activation energy (E_act) of the process of hydrolysis of casein and hemoglobin-lytic peptidases in the zone of low and high temperatures are different. In the presence of phenol, E_act values of the process of hydrolysis of casein casein-lytic peptidases at рН 7.4 increase; at рН 5.0 they decreases in the zone of high temperatures. The values of E_act of the process of hydrolysis of hemoglobin-lytic peptidases decrease in most cases. The mechanisms of the influence of phenol on peptidase activity and digestive processes in benthivorous fish feeding on chironomid larvae are discussed.

中文翻译：

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不同рН值下苯酚对拟幼虫肽酶活性和温度特性的影响。

摘要

苯酚（Ph，0.25 mmol / L）对在拟南芥幼虫Chironomus sp。的整个生物体中起作用的酪蛋白和血红蛋白溶解肽酶的活性和温度依赖性的影响。研究了0−70°С温度范围内在不同рН值（3.0、5.0和7.4）下的温度。拟虫幼虫肽酶的最适温度为40°С。在苯酚的影响下，酪蛋白分解肽酶的活性在20°С和рН5.0处显着增加（3.3倍），而血红蛋白分解肽酶的活性在рН3.0处（显着3.5倍）更显着。 。该效果从рН3.0逐渐降低到рН7.4。在两个温度下。在苯酚的存在下，温度依赖性和Q ₁₀的曲线形状值在高温区域变化最大。在低温和高温区域中酪蛋白和血红蛋白溶解肽酶水解过程的活化能（E _act）值是不同的。在苯酚的存在下，酪蛋白-酪蛋白水解肽酶水解过程的E _act值在рН7.4处增加；在рН5.0处，它们在高温区域降低。在大多数情况下，血红蛋白水解肽酶水解过程中的E _act值降低。讨论了苯酚对chi虫幼体底栖鱼类摄食过程中肽酶活性和消化过程的影响机理。