Abstract

New two-component pyrene probes based on oligo(2'-O-methylribonucleotides) for microRNA detection have been designed. They contain the ^PyA-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (3WJ) structure with a target RNA. We have chosen microRNA let-7a-3p as the RNA target because of the correlation of its concentration in cells with the appearance and progression of cancer. We have compared the thermal stability and fluorescence properties of the two-component probes based on oligo(2'-O-methylribonucleotides) that contain either deoxyriboadenosine (dAdA^PyAdAdA) or the (2'-O-methylribo)adenosine (A^mA^{m Py}AA^mA^m) cluster with those of (dAdA^PyAdAdA)-containing oligodeoxyribonucleotide. The changes in the fluorescence spectra of two-component probes after hybridization with the RNA target have been demonstrated. These probes can be used for designing a new microRNA detection system.

中文翻译：

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基于Oligo（2'-O-甲基核糖核苷酸）的新型双组分P探针用于microRNA检测

摘要

设计了基于寡核苷酸（2'- O-甲基核糖核苷酸）的新型两组分pyr探针。它们包含^Py A修饰的腺嘌呤簇（戊腺苷片段，其中心包含8-（1-乙炔基py）-脱氧核糖腺苷），并可与靶RNA形成三向连接（3WJ）结构。我们选择microRNA let-7a-3p作为RNA靶标是因为其在细胞中的浓度与癌症的发生和发展相关。我们已经比较了基于寡聚（2'- O-甲基核糖核苷酸）的两组分探针的热稳定性和荧光性质，该寡核苷酸包含脱氧核糖腺苷（dAdA ^Py AdAdA）或（2'- O-甲基核糖）腺苷（A^m A ^{m Py} AA ^m A ^m）簇与包含（dAdA ^Py AdAdA）的寡脱氧核糖核苷酸的簇。已经证明了与RNA靶杂交后两组分探针的荧光光谱的变化。这些探针可用于设计新的microRNA检测系统。