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Dynamics of apoptosis-related gene expression during follicular development in yak
Journal of Animal Physiology and Animal Nutrition ( IF 2.2 ) Pub Date : 2021-04-26 , DOI: 10.1111/jpn.13527
Taojie Zhang 1, 2 , Wang-Dui BaSang 3 , Weihua Chang 4 , Shengdong Huo 2 , Xingbin Ma 5 , Xianghong Ju 5 , Sijiu Yu 6 , Sheng Cui 1
Affiliation  

The potential reproduction power of domestic animals is limited by a complicated follicular atresia process. P53, caspase-9 (Casp9), Bax, Bcl-2 and Fas play a crucial role in the ovarian mitochondrion-dependent apoptosis and death receptor pathway. In accordance with this study, the expression levels of Casp9, Bax, Bcl-2 and Fas were analysed in ovaries and oviducts of yak by immunohistochemistry (IHC). P53 and the above in ovarian granulosa cells (GCs) from atretic (3–6 mm) to healthy follicles (6–8 mm) and in oviducts were examined from the luteal phase to the follicular phase during the oestrous circle by Western blot (WB) and real-time PCR (RT-PCR). Results demonstrated that typical classic apoptotic factors Casp9, Bax, Bcl-2 and Fas were expressed in the cytoplasm and zonal pellucida of oocytes, primordial follicles, primary follicles, ovarian surface epithelium, ovarian GCs, granular lutein cells, surface epithelia in oviduct uterotubal junction and oviduct ampulla during the luteal phase. RT-PCR and WB revealed that P53 and Fas significantly increased in GCs of atretic follicles. P53 and Casp9 increased in oviduct epithelium during the luteal phase, but Fas was unchanged. A contrary tendency was noted in Bcl-2 and Bax expression. Overall, P53 and Fas play an essential role in inducing GC apoptosis, and Bax, Bcl-2, Casp9 and P53 are involved in oviduct epithelial regeneration in yak.

中文翻译:

牦牛卵泡发育过程中凋亡相关基因的表达动态

复杂的卵泡闭锁过程限制了家养动物的潜在繁殖能力。P53、caspase-9 (Casp9)、Bax、Bcl-2 和 Fas 在卵巢线粒体依赖性细胞凋亡和死亡受体通路中起关键作用。根据这项研究,通过免疫组织化学(IHC)分析了牦牛卵巢和输卵管中 Casp9、Bax、Bcl-2 和 Fas 的表达水平。通过蛋白质印迹法(WB)检测了从闭锁(3-6 毫米)到健康卵泡(6-8 毫米)和输卵管中从黄体期到发情周期卵泡期的 P53 及以上) 和实时 PCR (RT-PCR)。结果表明,典型的经典凋亡因子 Casp9、Bax、Bcl-2 和 Fas 在卵母细胞、原始卵泡、初级卵泡、卵巢表面上皮、卵巢 GCs、颗粒状叶黄素细胞、黄体期输卵管子宫输卵管连接处和输卵管壶腹的表面上皮。RT-PCR 和 WB 显示 P53 和 Fas 在闭锁卵泡的 GC 中显着增加。P53 和 Casp9 在黄体期在输卵管上皮中增加,但 Fas 没有变化。在 Bcl-2 和 Bax 表达中注意到相反的趋势。总体而言,P53 和 Fas 在诱导 GC 细胞凋亡中起重要作用,Bax、Bcl-2、Casp9 和 P53 参与牦牛的输卵管上皮再生。P53 和 Casp9 在黄体期在输卵管上皮中增加,但 Fas 没有变化。在 Bcl-2 和 Bax 表达中注意到相反的趋势。总体而言,P53 和 Fas 在诱导 GC 细胞凋亡中起重要作用,Bax、Bcl-2、Casp9 和 P53 参与牦牛的输卵管上皮再生。P53 和 Casp9 在黄体期在输卵管上皮中增加,但 Fas 没有变化。在 Bcl-2 和 Bax 表达中注意到相反的趋势。总体而言,P53 和 Fas 在诱导 GC 细胞凋亡中起重要作用,Bax、Bcl-2、Casp9 和 P53 参与牦牛的输卵管上皮再生。
更新日期:2021-04-26
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