Super-resolution microscopy (SRM) imaging of the finite subcellular structures and subtle bioactivities inside organelles delivers abundant cellular information with high fidelity to unravel the intricate biological processes. An ideal fluorescent probe with precise control of fluorescence is critical in SRM technique like stimulated emission depletion (STED). Si-rhodamine was decorated with both targeting group and H⁺-receptor, affording the dually fluorogenic Si-rhodamine in which the NIR fluorescence was efficiently controlled by the coalescent of spirolactone-zwitterion equilibrium and PeT mechanism. The dually fluorogenic characters of the probe offer a perfect mutual enhancement in sensitivity, specificity and spatial resolution. Strong fluorescence only released in the existence of targeting protein at acidic lysosomal pH, ensured precisely tracking the dynamic of lysosomal structure and pH in living cells by STED.

中文翻译：

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STED 通过双荧光硅罗丹明对溶酶体的动力学进行成像

细胞器内有限亚细胞结构和微妙生物活性的超分辨率显微镜 (SRM) 成像可提供丰富的高保真细胞信息，以解开复杂的生物过程。具有精确控制荧光的理想荧光探针在受激发射损耗 (STED) 等 SRM 技术中至关重要。Si-罗丹明同时装饰有靶向基团和 H ⁺-受体，提供双荧光硅罗丹明，其中 NIR 荧光由螺内酯 - 两性离子平衡和 PeT 机制的聚结有效控制。探针的双重荧光特性在灵敏度、特异性和空间分辨率方面提供了完美的相互增强。只有在酸性溶酶体pH值存在靶向蛋白时才会释放强荧光，确保STED精确跟踪活细胞中溶酶体结构和pH值的动态。