Abstract—One of the priorities of neuroscience is the fight against socially relevant neurodegenerative diseases, including Parkinson’s disease (PD). Given the limited possibilities of studying the pathogenesis of this disease, the creation and detailed study of appropriate experimental models is of great importance. Therefore, the purpose of this work was to continue the long-term study of the cellular and molecular mechanisms of the pathogenesis of Parkinson’s disease and their systemic manifestations in a model of this disease in mice which is based on the use of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Here, using Western blotting, it was shown that in the blood plasma of mice with MPTP-induced clinical stage of PD, the ratio of monomeric to oligomeric α-synuclein is almost halved, and there is a tendency toward a decrease in the ratio of total α-synuclein to α-synuclein phosphorylated at serine-129. According to several studies, these changes are typical in patients with PD. Unlike plasma, no change in the ratio of these proteins was found in lymphocytes. From the alignment of our data on the content of α-synuclein and its isoforms in the blood of Parkinsonian mice, and the literature data on the content of α-synuclein in PD patients, it follows that our PD model can be used for an in-depth assessment of the peripheral manifestations of this disease, as well as for improving the differential diagnosis of PD, analyzing the effectiveness therapy, and screening for new drugs.

摘要—神经科学的优先事项之一是与社会相关的神经退行性疾病（包括帕金森氏病（PD））作斗争。由于研究这种疾病的发病机理的可能性有限，因此建立和详细研究合适的实验模型非常重要。因此，这项工作的目的是继续对小鼠帕金森氏病的发病机理及其系统性表现的细胞和分子机制进行长期研究，该模型基于1-甲基- 4-苯基-1,2,3,6-四氢吡啶（MPTP）。在这里，使用蛋白质印迹法显示，在MPTP诱导的PD临床阶段的小鼠血浆中，单体与寡聚α-突触核蛋白的比例几乎减半，并且存在在丝氨酸129处磷酸化的总α-突触核蛋白与α-突触核蛋白之比降低的趋势。根据数项研究，这些变化在PD患者中是典型的。与血浆不同，在淋巴细胞中未发现这些蛋白质比例的变化。从我们有关帕金森病小鼠血液中α-突触核蛋白及其同工型含量的数据的对比，以及有关PD患者的α-突触核蛋白含量的文献数据，可以得出结论，我们的PD模型可用于糖尿病的诊断。深入评估该疾病的外周表现，以及改善PD的鉴别诊断，分析有效性疗法和筛选新药。在淋巴细胞中未发现这些蛋白质比例的变化。从我们有关帕金森病小鼠血液中α-突触核蛋白及其同工型含量的数据的比对，以及有关PD患者的α-突触核蛋白含量的文献数据，可以得出结论，我们的PD模型可用于糖尿病的诊断。深入评估该疾病的外周表现，以及改善PD的鉴别诊断，分析有效性疗法和筛选新药。在淋巴细胞中未发现这些蛋白质比例的变化。从我们有关帕金森病小鼠血液中α-突触核蛋白及其同工型含量的数据的对比，以及有关PD患者的α-突触核蛋白含量的文献数据，可以得出结论，我们的PD模型可用于糖尿病的诊断。深入评估该疾病的外周表现，以及改善PD的鉴别诊断，分析有效性疗法和筛选新药。