ABSTRACT

Introduction

Rapid and accurate diagnostic approaches are essential for impeding the spread of infectious diseases. This review aims to summarize current progress of clustered regularly interspaced short palindromic repeats (CRISPR)‐associated (Cas) systems in the applications for diagnostics of infectious diseases including the ongoing COVID-19 epidemic.

Areas covered

In this review, we discuss class 2 CRISPR-Cas biosensing systems-based diagnostics in various emerging and reemerging infectious diseases, CRISPR-Cas systems have created a new era for early diagnostics of infectious diseases, especially with the discovery of the collateral cleavage activity of Cas12 and Cas13. We mainly focus on different CRISPR-Cas effectors for the detection of pathogenic microorganisms as well as provide a detailed explanation of the pros and cons of CRISPR-Cas biosensing systems. In addition, we also introduce future research perspectives.

Expert commentary

However, further improvement of newly discovered systems and engineering existing ones should be developed to increase the specificity, sensitivity or stability of the diagnostic tools. It may be a long journey to finish the clinical transition from research use. CRISPR-Cas approaches will emerge as more promising and robust tools for infectious disease diagnosis in the future.

中文翻译：

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CRISPR-Cas 系统在传染病诊断中的应用

摘要

介绍

快速准确的诊断方法对于阻止传染病的传播至关重要。本综述旨在总结成簇规则间隔短回文重复序列 (CRISPR) 相关 (Cas) 系统在传染病诊断应用中的当前进展，包括正在进行的 COVID-19 流行病。

覆盖区域

在这篇综述中，我们讨论了基于 CRISPR-Cas 生物传感系统在各种新出现和重新出现的传染病中的诊断，CRISPR-Cas 系统为传染病的早期诊断创造了一个新时代，特别是发现了Cas12 和 Cas13。我们主要关注用于检测病原微生物的不同 CRISPR-Cas 效应器，并详细解释 CRISPR-Cas 生物传感系统的优缺点。此外，我们还介绍了未来的研究前景。

专家评论

然而，应进一步改进新发现的系统和改造现有系统，以提高诊断工具的特异性、敏感性或稳定性。完成从研究用途的临床过渡可能是一个漫长的旅程。CRISPR-Cas 方法将在未来成为更有前途和更强大的传染病诊断工具。