In the present work, we study the ovule, seed, and fruit development in six Bulbostylis species in order to characterize the genus in a comparative approach and to identify the characteristics that can be used in taxonomy and phylogeny. Flowers and fruits at different developmental stages were analyzed using LM and SEM after processing according to standard techniques. The species studied have the following: anatropous and bitegmic ovules, weak crassinucellar ovules, obturator of integumentary origin, monosporic embryo sac of the Polygonum type, nuclear endosperm, hypostase formation, seed coat formed by tanniferous endotegmen and exotesta, and Bulbostylis-type embryo. On the other hand, the pericarp development constitutes the main variation within Bulbostylis since the cells of the exocarp may or may not present starch grains, and their inner periclinal walls may be slightly or deeply concave depending on the degree of development of the mesocarp sclereids. In a taxonomic context, the results herein obtained are in conflict with studies which suggest infrageneric groupings based on fruit micromorphology, and also with the relationship among the Bulbostylis species based on molecular analysis. This work contributes to a better understanding of the reproductive anatomy and embryology in Bulbostylis, and reveals the first insights about the origin of multiple embryos in Cyperaceae. Given the frequent presence of polyembryony in Bulbostylis, and the poor mention of this condition in the family, this work highlights an aspect in the anatomy of Cyperaceae that must be re-explored.

在目前的工作中，我们研究了六种球茎属物种的胚珠、种子和果实发育，以便通过比较方法表征该属并确定可用于分类学和系统发育的特征。根据标准技术处理后，使用 LM 和 SEM 分析不同发育阶段的花和果实。所研究的物种有以下几种：倒生和双胚珠、弱粗胚珠、外皮来源的闭孔、蓼型的单孢胚囊、核胚乳、原位酶形成、由鞣质内胚层和外胚层形成的种皮和球茎型胚。另一方面，果皮发育构成了内部的主要变异。Bulbostylis，因为外果皮的细胞可能存在也可能不存在淀粉粒，并且它们的内周壁可能略微或深凹，这取决于中果皮硬核的发育程度。在分类学背景下，本文获得的结果与基于果实微形态学的亚属分组的研究以及基于分子分析的球茎属物种之间的关系的研究相矛盾。这项工作有助于更好地了解球茎菌的生殖解剖学和胚胎学，并首次揭示莎草科多个胚胎的起源。鉴于球茎菌中经常存在多胚，以及在家庭中很少提及这种情况，这项工作突出了莎草科解剖学中必须重新探索的一个方面。