Redistribution of FLAgellar Member 8 during the trypanosome life cycle: Consequences for cell fate prediction,Cellular Microbiology

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Redistribution of FLAgellar Member 8 during the trypanosome life cycle: Consequences for cell fate prediction
Cellular Microbiology ( IF 2.6 ) Pub Date : 2021-04-25 , DOI: 10.1111/cmi.13347
Estefanía Calvo-Álvarez _{1,

2} , Serge Bonnefoy ₁ , Audrey Salles ₃ , Fiona E Benson ₄ , Paul G McKean ₄ , Philippe Bastin ₁ , Brice Rotureau _{1,

2}

Affiliation

The single flagellum of African trypanosomes is essential in multiple aspects of the parasites' development. The FLAgellar Member 8 protein (FLAM8), localised to the tip of the flagellum in cultured insect forms of Trypanosoma brucei, was identified as a marker of the locking event that controls flagellum length. Here, we investigated whether FLAM8 could also reflect the flagellum maturation state in other parasite cycle stages. We observed that FLAM8 distribution extended along the entire flagellar cytoskeleton in mammalian-infective forms. Then, a rapid FLAM8 concentration to the distal tip occurs during differentiation into early insect forms, illustrating the remodelling of an existing flagellum. In the tsetse cardia, FLAM8 further localises to the entire length of the new flagellum during an asymmetric division. Strikingly, in parasites dividing in the tsetse midgut and in the salivary glands, the amount and distribution of FLAM8 in the new flagellum were seen to predict the daughter cell fate. We propose and discuss how FLAM8 could be considered a meta-marker of the flagellum stage and maturation state in trypanosomes.

中文翻译：

锥虫生命周期中 FLAgellar 成员 8 的重新分布：细胞命运预测的后果

非洲锥虫的单个鞭毛在寄生虫发育的多个方面是必不可少的。FLAgellar 成员 8 蛋白 (FLAM8)，定位于培养的布氏锥虫形式的鞭毛尖端，被确定为控制鞭毛长度的锁定事件的标记。在这里，我们研究了 FLAM8 是否也可以反映其他寄生虫周期阶段的鞭毛成熟状态。我们观察到 FLAM8 分布沿哺乳动物感染形式的整个鞭毛细胞骨架延伸。然后，在分化为早期昆虫形式的过程中，远端尖端的快速 FLAM8 浓度发生，说明现有鞭毛的重塑。在采采蝇贲门中，FLAM8 在不对称分裂期间进一步定位于新鞭毛的整个长度。引人注目的是，在采采蝇中肠和唾液腺中分裂的寄生虫中，新鞭毛中 FLAM8 的数量和分布可以预测子细胞的命运。

更新日期：2021-04-25

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