Octocrylene (OC) is a broad-spectrum ultraviolet-absorbing chemical used in sunscreen and other personal care products. Its health effects are a concern because it has been detected in water, fish, humans, and food chains. In vivo and in vitro investigations were performed in zebrafish (Danio rerio) larvae and a zebrafish liver cell line (ZFL), respectively, to understand the potential risks and molecular mechanisms of OC toxicity. The 96-h median lethal concentration (LC₅₀) of OC was determined to be 251.8 μM in larvae and 5.5 μM in ZFL cells. Quantitative real-time PCR (qRT-PCR) showed that OC induced the expression of genes for CYPs (CYP1A, CYP3A65), estrogen receptors (ERα, ERβ1, GPER), vitellogenin (VTG1), and sex determination (BRCA2, CYP19A, DMRT1, SOX9A), both in vitro and in vivo. A whole-transcriptome sequencing method was used to evaluate the gene expression profile of larvae exposed to OC. OC was found to mediate the biosynthesis of estrogens (such as estriol) and affect the antioxidant pathway (glutathione transferases and peroxisome). These findings clarify the toxic effects and molecular mechanisms of OC and support banning its use in cosmetics.

辛二烯（OC）是一种广谱紫外线吸收化学品，用于防晒霜和其他个人护理产品中。它的健康影响值得关注，因为已经在水，鱼类，人类和食物链中发现了它。分别在斑马鱼（Danio rerio）幼虫和斑马鱼肝细胞系（ZFL）中进行了体内和体外研究，以了解OC毒性的潜在风险和分子机制。96小时平均致死浓度（LC ₅₀在幼虫中的OC含量测定为251.8μM，在ZFL细胞中的OC测定为5.5μM。实时定量PCR（qRT-PCR）显示，OC诱导了CYP（CYP1A，CYP3A65），雌激素受体（ERα，ERβ1，GPER），卵黄蛋白原（VTG1）和性别决定（BRCA2，CYP19A，DMRT1）基因的表达，SOX9A），两者都在体外和体内。使用全转录组测序方法评估暴露于OC的幼虫的基因表达谱。OC被发现可以介导雌激素（例如雌三醇）的生物合成并影响抗氧化剂途径（谷胱甘肽转移酶和过氧化物酶体）。这些发现阐明了OC的毒性作用和分子机理，并支持禁止在化妆品中使用它。