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Detection and genotyping of Listeria monocytogenes in artisanal soft cheeses from Ecuador
Revista Argentina de Microbiología ( IF 1.9 ) Pub Date : 2021-04-24 , DOI: 10.1016/j.ram.2021.02.013
Estefania Espinosa-Mata 1 , Lorena Mejía 1 , José E Villacís 2 , Viviana Alban 3 , Sonia Zapata 1
Affiliation  

Listeria monocytogenes is one of the most important bacteria associated with foodborne diseases, soft cheese being an important L. monocytogenes vehicle. In Ecuador, soft cheese is consumed in 84.3% of urban households. We determined the prevalence of L. monocytogenes and serogroups in 260 fresh artisanal soft cheese samples collected in 18 of 24 Ecuadorian provinces. Listeria spp. detection was carried out by culture-dependent and independent methods; 14.23% of samples were positive for L. monocytogenes. Serogroup IVb was found in 83.78% of the food isolates. Serogroups IIb and IIa were present in 8.11% of our isolates. To our knowledge, this is the first report of L. monocytogenes serogroups associated with food in Ecuador; we also found serogroup similarities between cheese isolates and clinical isolates.



中文翻译:

厄瓜多尔手工软奶酪中单核细胞增生李斯特菌的检测和基因分型

单核细胞增生李斯特菌是与食源性疾病相关的最重要的细菌之一,软奶酪是一种重要的单核细胞增生李斯特菌载体。在厄瓜多尔,84.3% 的城市家庭食用软奶酪。我们确定了在厄瓜多尔 24 个省份中的 18 个收集的 260 个新鲜手工软奶酪样品中单核细胞增生李斯特菌和血清群的流行率。李斯特菌属_ 通过培养依赖和独立的方法进行检测;14.23% 的样本呈单核细胞增生李斯特菌阳性。在 83.78% 的食物分离物中发现了血清组 IVb。血清组 IIb 和 IIa 存在于我们的分离株中 8.11%。据我们所知,这是L. monocytogenes的第一份报告厄瓜多尔与食物相关的血清群;我们还发现了奶酪分离株和临床分离株之间的血清群相似性。

更新日期:2021-04-24
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