To our knowledge, there was no study on interactive relationship between CHO molecular structure spectral profiles of newly developed cool-season adapted faba bean and nutritional characteristics in ruminants. The aim of this study was to evaluate the impact of genotypes and tannin levels on the physicochemical and nutritional characteristics of faba bean as an alternative protein and energy source for ruminants and its relation to CHO molecular structure spectral profiles using vibrational molecular spectroscopy (Ft/IR-ATR). Eight genotypes with two tannin levels (low and normal) grown at three different locations in Saskatchewan (CDC crop research fields) were analysed. Chemical analyses were performed using AOAC standards and energy was evaluated using NRC standards. Rumen degradation kinetics were determined using in situ dairy trial. Intestinal digestion was analysed by a modified three-step in vitro technique with 12 h pre-rumen incubation in dairy cows. Molecular spectral study was performed using Ft/IR–ATR, and the molecular structural features were analysed at ca. 4000–800 cm⁻¹ using the Spectra ManagerII. CHO-related functional groups were analysed with OMNIC 7.3. The results showed low-tannin faba bean (LT) presented a highly significant difference (p < 0.05) on rumen bypass starch (BSt) compared to normal tannin faba bean (NT). On the other hand, NT had more total tract digested starch (TDST) compared to LT. No significant differences (p > 0.10) were observed for total digestible nutrients (TDN_1x), metabolizable protein (MP), feed milk value (FMV) or rumen undegraded crude protein (RUP). Results showed differences in physicochemical characteristics among faba bean varieties; however, the predicted production performance was not different within faba bean genotypes. These outcomes suggest that faba bean can be used as nutritive ingredient for dairy cattle without a significant genotype or tannin level effect on metabolic characteristics. Results from vibrational spectroscopic study showed a higher ATR-Ft/IR absorbance (p < 0.05) in NT on inherent structural CHO (STCHO), total CHO (TCHO) area and peaks (H_1015, H_1076, H_1145), and cellulosic compounds (CEC) to total CHO (TCHO) ratio. Significant correlations could be found between CHO spectral profiles and metabolic characteristics of faba bean, which indicates that structural spectral features of faba bean could be used to predict metabolic characteristics in ruminants.

中文翻译：

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使用标准方法和振动分子光谱 (Ft/IR-ATR) 分析与新开发的低单宁和正常单宁蚕豆品种奶牛营养特性相关的碳水化合物分子结构谱

据我们所知，目前还没有研究新开发的适应冷季蚕豆的CHO分子结构光谱图与反刍动物营养特性之间的交互关系。本研究的目的是评估基因型和单宁水平对蚕豆作为反刍动物替代蛋白质和能源的理化和营养特性的影响及其与使用振动分子光谱 (Ft/IR) 的 CHO 分子结构光谱图谱的关系-ATR)。分析了在萨斯喀彻温省三个不同地点（CDC 作物研究领域）生长的具有两种单宁水平（低和正常）的八种基因型。使用 AOAC 标准进行化学分析，使用 NRC 标准评估能量。瘤胃降解动力学使用原位乳品试验。通过改进的三步体外技术分析肠道消化，在奶牛中进行 12 小时的瘤胃前孵育。使用 Ft / IR-ATR 进行分子光谱研究，并在 ca 分析分子结构特征。4000–800 cm ^-1使用 Spectra ManagerII。使用 OMNIC 7.3 分析 CHO 相关功能组。结果表明，与普通单宁蚕豆（NT）相比，低单宁蚕豆（LT）在瘤胃旁路淀粉（BSt）上的差异非常显着（p  < 0.05）。另一方面，与 LT 相比，NT 具有更多的全消化道消化淀粉 (TDST)。_{总可消化营养素 (TDN 1x} ) 未观察到显着差异 ( p  > 0.10))、可代谢蛋白 (MP)、饲料乳值 (FMV) 或瘤胃未降解粗蛋白 (RUP)。结果表明蚕豆品种理化特性存在差异；然而，预测的生产性能在蚕豆基因型中没有不同。这些结果表明蚕豆可用作奶牛的营养成分，而对代谢特征没有显着的基因型或单宁水平影响。振动光谱研究结果显示更高的 ATR-Ft/IR 吸光度 ( p < 0.05) 在 NT 中固有结构 CHO (STCHO)、总 CHO (TCHO) 面积和峰（H_1015、H_1076、H_1145）以及纤维素化合物 (CEC) 与总 CHO (TCHO) 的比率。CHO光谱图与蚕豆代谢特征之间存在显着相关性，表明蚕豆的结构光谱特征可用于预测反刍动物的代谢特征。