Objective

The objective was to apply the silver nanoparticles (AgNPs) in situ synthesized in an adhesive resin.

Materials and methods

Silver 2-ethylhexanoate was added into Clearfil SE Bond (Kuraray Inc.) to form 0.0% Ag, 0.1% Ag, 0.2% Ag groups. The size and distribution of AgNPs formed in light-cured adhesive resin disks were observed by TEM. The degree of conversion was evaluated by FTIR. The cytotoxicity against human periodontal ligament fibroblasts was examined by CCK-8 assay. After each water-storage time (1 week, 4 weeks, 3 months, 12 months), biofilms of Streptococcus mutans on the resin disks were observed by SEM and tested for live/dead staining and colony-forming units.

Results

The average particle sizes of evenly dispersed AgNPs in the 0.1% Ag and 0.2% Ag groups were 10.9 ± 3.7 nm and 8.8 ± 4.2 nm, respectively. The incorporation of AgNPs did not compromise the degree of conversion and biocompatibility of the 0.1% Ag and 0.2% Ag groups. After 12 months of water storage, the antibacterial properties of 0.1% Ag and 0.2% Ag groups were much higher than that of 0.0% Ag group.

Conclusion

The in situ synthesis of AgNPs might be a promising technique in the antibacterial modification of dental adhesive resin.

中文翻译：

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银纳米粒子原位合成在牙科胶粘剂中的应用

客观的

目的是应用在粘合剂树脂中原位合成的银纳米颗粒（AgNPs）。

材料和方法

将2-乙基己酸银添加到Clearfil SE Bond（Kuraray Inc.）中以形成0.0％Ag，0.1％Ag，0.2％Ag基团。通过TEM观察在光固化的粘合树脂盘中形成的AgNP的尺寸和分布。通过FTIR评估转化度。通过CCK-8测定法检查了对人牙周膜成纤维细胞的细胞毒性。在每个储水时间（1周，4周，3个月，12个月）后，通过SEM观察树脂盘上变异链球菌的生物膜，并测试活/死染色和菌落形成单位。

结果

0.1％Ag和0.2％Ag组中均匀分散的AgNP的平均粒径分别为10.9±3.7 nm和8.8±4.2 nm。AgNP的掺入不损害0.1％Ag和0.2％Ag基团的转化度和生物相容性。贮水12个月后，0.1％Ag和0.2％Ag组的抗菌性能大大高于0.0％Ag组。

结论

AgNPs的原位合成可能是牙科粘合树脂抗菌改性的一种有前途的技术。