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DNA tetrahedron-mediated immune-sandwich assay for rapid and sensitive detection of PSA through a microfluidic electrochemical detection system
Microsystems & Nanoengineering ( IF 7.3 ) Pub Date : 2021-04-25 , DOI: 10.1038/s41378-021-00258-x
Dezhi Feng 1, 2, 3 , Jing Su 4 , Yi Xu 2 , Guifang He 2, 5 , Chenguang Wang 2, 3 , Xiao Wang 2, 5 , Tingrui Pan 6 , Xianting Ding 4 , Xianqiang Mi 1, 2, 3, 7, 8
Affiliation  

Prostate-specific antigen (PSA) is the most widely used biomarker for the early diagnosis of prostate cancer. Existing methods for PSA detection are burdened with some limitations and require improvement. Herein, we developed a novel microfluidic–electrochemical (μFEC) detection system for PSA detection. First, we constructed an electrochemical biosensor based on screen-printed electrodes (SPEs) with modification of gold nanoflowers (Au NFs) and DNA tetrahedron structural probes (TSPs), which showed great detection performance. Second, we fabricated microfluidic chips by DNA TSP-Au NF-modified SPEs and a PDMS layer with designed dense meandering microchannels. Finally, the μFEC detection system was achieved based on microfluidic chips integrated with the liquid automatic conveying unit and electrochemical detection platform. The μFEC system we developed acquired great detection performance for PSA detection in PBS solution. For PSA assays in spiked serum samples of the μFEC system, we obtained a linear dynamic range of 1–100 ng/mL with a limit of detection of 0.2 ng/mL and a total reaction time <25 min. Real serum samples of prostate cancer patients presented a strong correlation between the “gold-standard” chemiluminescence assays and the μFEC system. In terms of operation procedure, cost, and reaction time, our method was superior to the current methods for PSA detection and shows great potential for practical clinical application in the future.



中文翻译:

DNA四面体介导的免疫夹心法通过微流体电化学检测系统快速灵敏地检测PSA

前列腺特异性抗原(PSA)是前列腺癌早期诊断中使用最广泛的生物标志物。现有的 PSA 检测方法存在一些局限性,需要改进。在此,我们开发了一种用于 PSA 检测的新型微流体电化学 (μFEC) 检测系统。首先,我们构建了一种基于丝网印刷电极(SPEs)的电化学生物传感器,修饰了金纳米花(Au NFs)和 DNA 四面体结构探针(TSPs),表现出良好的检测性能。其次,我们通过 DNA TSP-Au NF 修饰的 SPE 和具有设计密集曲折微通道的 PDMS 层制造了微流控芯片。最后,实现了基于微流控芯片与液体自动输送单元和电化学检测平台集成的μFEC检测系统。我们开发的 μFEC 系统在 PBS 溶液中的 PSA 检测中获得了出色的检测性能。对于 μFEC 系统的加标血清样品中的 PSA 分析,我们获得了 1–100 ng/mL 的线性动态范围,检测限为 0.2 ng/mL,总反应时间 <25 分钟。前列腺癌患者的真实血清样本显示出“金标准”化学发光检测与 μFEC 系统之间的强相关性。在操作程序、成本和反应时间方面,我们的方法优于现有的 PSA 检测方法,在未来的实际临床应用中显示出巨大的潜力。2 ng/mL,总反应时间 <25 分钟。前列腺癌患者的真实血清样本显示出“金标准”化学发光检测与 μFEC 系统之间的强相关性。在操作程序、成本和反应时间方面,我们的方法优于现有的 PSA 检测方法,在未来的实际临床应用中显示出巨大的潜力。2 ng/mL,总反应时间 <25 分钟。前列腺癌患者的真实血清样本显示出“金标准”化学发光检测与 μFEC 系统之间的强相关性。在操作程序、成本和反应时间方面,我们的方法优于现有的 PSA 检测方法,在未来的实际临床应用中显示出巨大的潜力。

更新日期:2021-04-26
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