Urginea sanguinea, a detoxifier utilised in African herbal medicine, contains toxic cardiac glycosides that are considered to have anticancer properties. In this study, manganese oxide nanoparticles (MnO_xNPs) were synthesised using Urginea sanguinea and its isolates, a homoisoflavone (urgineanin E) and a mixture of bufadienolides (proscillaridin A, scillaren A and 5α-4,5-dihydroscillaren A), and their cytotoxic effects and antioxidant activities were investigated. The presence of absorption peaks between 262 nm and 272 nm using ultraviolet-visible spectroscopy, and Mn-O-Mn vibrations in the infrared spectrum confirmed the formation of MnO_xNPs. Images from high-resolution transmission electron microscopy and scanning electron microscopy revealed interconnected spheroidal shapes with nanorods for MnO_xNPs (extract), and ultrathin nanosheets assembled as nanoflowers for MnO_xNPs (urgineanin E) and MnO_xNPs (bufadienolides). The synthesised nanoparticles were evaluated for their anticancer properties using the MTT assay. The cytotoxic effects of MnO_xNPs against A549 and HepG2 cancer cell lines increased with increasing concentration with no significant cytotoxicity towards HEK293 normal cells. MnO_xNPs were found to exhibit moderate free radical scavenging activity. This warrants further research into the use of manganese oxide nanoparticles synthesised with medicinal plants and active compounds as anticancer agents.

Urginea sanguinea 是一种用于非洲草药的解毒剂，含有被认为具有抗癌特性的有毒强心苷。在这项研究中，氧化锰纳米粒子 (MnO _x NPs) 是使用Urginea sanguinea及其分离物、高异黄酮 (urgineanin E) 和蟾蜍二烯内酯 (proscillaridin A、scillaren A 和 5 α -4,5- dihydroscillaren A) 的混合物合成的，并对它们的细胞毒作用和抗氧化活性进行了研究。使用紫外-可见光谱在 262 nm 和 272 nm 之间存在吸收峰，红外光谱中的 Mn-O-Mn 振动证实了 MnO _x的形成NP。来自高分辨率透射电子显微镜和扫描电子显微镜的图像显示，MnO _x NPs（提取物）的纳米棒呈相互连接的球形，MnO _x NPs（urgineanin E）和MnO _x NPs（bufadienolides）的超薄纳米片组装为纳米花。使用 MTT 测定法评估合成的纳米粒子的抗癌特性。MnO _x NPs 对 A549 和 HepG2 癌细胞系的细胞毒性作用随着浓度的增加而增加，而对 HEK293 正常细胞没有显着的细胞毒性。MnO的_X发现 NPs 表现出中等的自由基清除活性。这需要进一步研究使用药用植物和活性化合物合成的氧化锰纳米粒子作为抗癌剂。