Rapid detection of small amounts of Staphylococcus aureus (S. aureus) in whole blood and the corresponding diagnosis of S. aureus is a complicated and time-consuming process. Gold standard is to culture and grow bacteria before detection, which typically takes several days and requires skilled personnel. Here, we developed a microfluidic cartridge for the culture-free isolation of S. aureus in blood and diagnosed them using a multiplex polymerase chain reaction (PCR) method. Isolation of S. aureus was achieved from large volumes of blood (typically of the order of 5 ml) using Apolipoprotein H coated magnetic beads followed by ultrasonication, a similar kind of agitation utilized in an ultrasonic bath, to lyse the enriched bacteria. The designed cartridge consisted of a rotary valve to control the sample flow and, also to direct the samples to the designated chambers for the pre-processing and bacteria isolation. By comparing PCR results from batch process and cartridge based method, we proved that the current cartridge design was capable of S. aureus detection in whole blood sample.

快速检测全血中的少量金黄色葡萄球菌（S. aureus）并相应诊断金黄色葡萄球菌是一个复杂且耗时的过程。黄金标准是在检测前培养和生长细菌，这通常需要几天时间并且需要熟练的人员。在这里，我们开发了一种微流体墨盒，用于血液中金黄色葡萄球菌的无培养分离，并使用多重聚合酶链反应 (PCR) 方法对其进行诊断。金黄色葡萄球菌的分离使用载脂蛋白 H 包被的磁珠从大量血液（通常约为 5 毫升）中获得，然后进行超声波处理，这是一种在超声波浴中使用的类似搅拌，以裂解富集的细菌。设计的滤芯由一个旋转阀组成，用于控制样品流量，并将样品引导至指定的腔室进行预处理和细菌分离。通过比较批处理和基于试剂盒的方法的 PCR 结果，我们证明当前的试剂盒设计能够检测全血样本中的金黄色葡萄球菌。