Aqueous glutathione selenium nano-incorporation (GSH-SeN-Inco) was prepared by gamma radiation in presence of microbial glutathione (GSH) and selenium dioxide. The novel prepared GSH-SeN-Inco are validated by UV–vis spectroscopy, TEM (17.5 nm), DLS, XRD, EDX and FTIR spectrum reveals the presence of GSH moiety that coating the selenium nanoparticles (SeNPs) forming GSH-SeN-Inco. The XRD analysis verified the presence of metallic SeNPs. The nucleation and radiolysis mechanism of GSH-SeN-Inco formation are also discussed. The size GSH-SeN-Inco (17.5 nm) is affected by certain factors such as concentration of GSH, selenium dioxide, and absorbed dose of gamma radiation. The present study explored the positive role of GSH-SeN-Inco as an antitumor activity against HepG-2 and MCF-7, with IC₅₀ at a concentration of 1.00 and 0.9 mM, respectively. The GSH-SeN-Inco show significant scavenging activity at 33%. The GSH-SeN-Inco shows antimicrobial potential against Gram-negative and Gram-positive bacteria with significant MIC especially Escherichia coli ATCC 25922 at 5.20 µg/ml.

在微生物谷胱甘肽 (GSH) 和二氧化硒存在下通过伽马辐射制备水性谷胱甘肽硒纳米掺入物 (GSH-SeN-Inco)。新制备的 GSH-SeN-Inco 经紫外-可见光谱、TEM (17.5 nm)、DLS、XRD、EDX 和 FTIR 光谱验证，揭示了 GSH 部分的存在，该部分包覆了形成 GSH-SeN-Inco 的硒纳米颗粒 (SeNPs) . XRD分析证实了金属SeNPs的存在。还讨论了GSH-SeN-Inco形成的成核和辐射分解机制。GSH-SeN-Inco (17.5 nm) 的尺寸受某些因素的影响，例如 GSH 的浓度、二氧化硒和伽马辐射的吸收剂量。本研究探讨了 GSH-SeN-Inco 作为抗 HepG-2 和 MCF-7 的抗肿瘤活性的积极作用，IC ₅₀浓度分别为 1.00 和 0.9 mM。GSH-SeN-Inco 在 33% 时显示出显着的清除活性。GSH-SeN-Inco 对革兰氏阴性菌和革兰氏阳性菌具有抗菌潜力，具有显着的 MIC，尤其是5.20 µg/ml 的大肠杆菌ATCC 25922。