Abstract

Non-esterified fatty acids (NEFAs) and Insulin-like growth factor-I (IGF-1) concentrations are modified after the induction of a "male effect". The present study examined the effect of the introduction of males into a group of females that were previously isolated from males, during different phases of the sexual cycle, to determine the changes to the NEFAs and IGF-1 concentrations. Sixty-four does were divided into six groups. The males were introduced with the females at different points after sponge removal. Introduction was carried out 48 h (n = 10, Group 48H), 72 h (n = 12, Group 72H), 4 days (n = 10, Group 4 D), 13 days (n = 10, Group 13 D) or 18 days after sponge removal (n = 10, Group 18 D), and a control group was implemented that had no contact with males (n = 12, Control Group). Plasma samples were taken every day to determine daily progesterone (P4) and NEFAs concentrations, and every second day for IGF-1 determination. No effects from the experimental groups were observed on the mean NEFAs or IGF-1 concentrations (p > .05). No differences between the time before male introduction and after male introduction were observed on the NEFAs concentrations (p > .05) or IGF-1 concentrations (p > .05). On the whole, only differences were observed in the NEFAs concentrations between the follicular and the luteal phases (9.48 ± 0.38 vs 8.15 ± 0.15 mg/dL for follicular and luteal phases, respectively, p < .01). The results of the present experiment demonstrated that the introduction of sexually active males at different moments of the oestrous cycle does not modify the NEFAs or the IGF-1 concentrations.

• Highlights

• Results shows that NEFAs or IGF-1 concentrations did not vary after the introduction of males.

• Results shows that NEFAs concentrations increases during the follicular phase.

• Results shows that IGF-1 concentrations did not vary during the oestrous cycle.

摘要

诱导“男性效应”后，非酯化脂肪酸（NEFAs）和胰岛素样生长因子-I（IGF-1）的浓度会发生变化。本研究检查了在性周期的不同阶段将雄性引入一组先前与雄性分离的雌性中的作用，以确定NEFA和IGF-1浓度的变化。64个确实分为六个组。去除海绵后，在不同的位置将雄性与雌性一起引入。引入进行48小时（n = 10，组48H），72小时（n = 12，组72H），4天（n = 10，组4 D），13天（n = 10，组13 D）或去除海绵后18天（n = 10，组18 D），实施了与男性没有接触的对照组（n = 12，对照组）。每天采集血浆样品，以测定每日孕酮（P4）和NEFAs浓度，每隔两天采集一次，以测定IGF-1。未观察到实验组对平均NEFA或IGF-1浓度的影响（p  > .05）。在男性引入之前和男性引入之后的时间之间，在NEFAs浓度（p  > .05）或IGF-1浓度（p  > .05）上没有观察到差异。总体而言，在卵泡期和黄体期之间仅观察到NEFAs浓度的差异（卵泡期和黄体期分别为9.48±0.38和8.15±0.15 mg / dL，p  <.01）。本实验的结果表明，在雌性周期的不同时刻引入性活跃的雄性不会改变NEFA或IGF-1的浓度。

• 强调

• 结果显示，引入雄性后，NEFA或IGF-1浓度没有变化。

• 结果显示，在卵泡期，NEFAs浓度增加。

• 结果表明，IGF-1的浓度在发情周期中没有变化。