ABSTRACT

Metagenomics is a contemporary discipline in science that introduces a new horizon in biomolecular and genetic studies and provides a standard method for understanding biological diversity. In this context, we can only assess the functional potential of a certain community through metagenomics, but by understanding the metaphenomics, we can assign the functional genetic potential with respect to environmental resources. Metaphenomics studies the phenome of soil, plants or other organisms through a meta-analysis with the primary goal of establishing dose–response relationships across a wide range of phenotypic characteristics and abiotic environmental factors. Rather than conjecturing gene functions by metagenomes, the metaphenomic approaches can assist in understanding the substantial functioning of the microbiome. Coupling metagenomics with metaphenomics has the potential to overcome practical challenges in agriculture and ecological sustainability. On the grounds, this review was performed to present a deep understanding of soil metagenomics linked with metaphenomics, i.e. the phenotypic response of the soil microbiome. It also discusses how metagenomic applications and basic methods help study the phenotypic response of uncultured soil microbiomes to external factors. Metaphenomics can apply an untargeted multiomics approach to decipher the soil microbiome metaphenotypic response to environmental perturbations, and its application is also reviewed in this article.

摘要

宏基因组学是一门当代科学学科，它为生物分子和遗传研究引入了新的视野，并为理解生物多样性提供了标准方法。在这种情况下，我们只能通过宏基因组学来评估某个群落的功能潜力，但通过了解元表型组学，我们可以分配与环境资源相关的功能遗传潜力。元表型组学通过荟萃分析研究土壤、植物或其他生物的表型组，其主要目标是在广泛的表型特征和非生物环境因素之间建立剂量-反应关系。宏基因组方法不是通过宏基因组推测基因功能，而是有助于理解微生物组的实质功能。将宏基因组学与元表型组学相结合有可能克服农业和生态可持续性方面的实际挑战。因此，本综述旨在深入了解与元表型组学相关的土壤宏基因组学，即土壤微生物组的表型反应。它还讨论了宏基因组应用和基本方法如何帮助研究未培养的土壤微生物组对外部因素的表型反应。元表型组学可以应用非靶向多组学方法来破译土壤微生物组对环境扰动的元表型响应，本文还对其应用进行了综述。这篇综述旨在深入了解与元表型组学相关的土壤宏基因组学，即土壤微生物组的表型反应。它还讨论了宏基因组应用和基本方法如何帮助研究未培养的土壤微生物组对外部因素的表型反应。元表型组学可以应用非靶向多组学方法来破译土壤微生物组对环境扰动的元表型响应，本文还对其应用进行了综述。这篇综述旨在深入了解与元表型组学相关的土壤宏基因组学，即土壤微生物组的表型反应。它还讨论了宏基因组应用和基本方法如何帮助研究未培养的土壤微生物组对外部因素的表型反应。元表型组学可以应用非靶向多组学方法来破译土壤微生物组对环境扰动的元表型响应，本文还对其应用进行了综述。