ABSTRACT

In this study, the acute impacts of aqueous methoxychlor exposure on the viability, locomotive behavior, and heartrate of Daphnia magna was assessed. Adults were exposed to 0 (ethanol control), 1, 2.5, 5, or 10 µg/L of methoxychlor for 48 hr using a static exposure assay. After 24 hr mobility was quantified using automated behavioral analysis software and differences in mortality and heartrate assessed after 24 and 48 hr. Exposure caused an increase in mortality after 48 hr, with effects seen in as little as 24 hr in the 10 µg/L treatment. Decreases in speed, total distance traveled, and frozen events were observed after 24 hr. Heartrate in the 10 µg/L treatment was depressed after 24 hr, with decreases in the 2.5 and 5 µg/L treatments observed after 48 hr. These results provide an expanded understanding of the impacts of a well-established legacy organochlorine pesticide on a model invertebrate.

摘要

在这项研究中，暴露于甲氧氯水溶液对水蚤的生存能力，机车行为和心率的急性影响被评估。使用静态暴露测定法，将成年人暴露于0（乙醇对照），1、2.5、5或10 µg / L甲氧基氯中48小时。24小时后，使用自动行为分析软件对移动性进行量化，并在24和48小时后评估死亡率和心率差异。暴露会在48小时后导致死亡率增加，而在10 µg / L的处理中，暴露会在24小时之内见效。24小时后，速度降低，总行驶距离降低，并观察到冰冻事件。24小时后，10 µg / L处理的心率下降，48小时后，2.5和5 µg / L处理的心率下降。这些结果提供了对完善的传统有机氯农药对无脊椎动物模型的影响的广泛理解。