Comparison of Sustainable Lipid and Protein Removal Methods for the Isolation of Insect Chitin from Black Soldier Fly Exoskeleton,ACS Food Science & Technology

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Comparison of Sustainable Lipid and Protein Removal Methods for the Isolation of Insect Chitin from Black Soldier Fly Exoskeleton
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-04-22 , DOI: 10.1021/acsfoodscitech.0c00104
Yun Nian Tan ₁ , Yi Ling Chin ₁ , Wei Ning Chen ₁

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Black soldier fly (Hermetia illucens), an insect known for feeding on waste biomass and converting it into useful nutrients such as proteins, lipids, and chitin, has been reared on insect farms on a large scale. In this study, chitin was isolated from the exoskeleton of the black soldier fly using different extraction protocols, such as chemical solvents, enzyme-assisted fractionation, and supercritical carbon dioxide extraction. The levels of effectiveness of the recovery of the chitin fraction using different extraction methods were then evaluated by determining the physicochemical properties of the extracted insect chitin using Fourier transform infrared spectroscopy, elemental analysis, and X-ray diffraction. The results show that the use of eco-friendly acids and solvents such as lactic acid, acetic acid, and ethanol as well as bacterial proteases holds promise for the defatting, demineralization, and deproteinization of the exoskeleton to yield good quality chitin, albeit with impurities.

中文翻译：

从黑兵苍蝇外骨骼分离甲壳质昆虫的可持续脂质和蛋白质去除方法的比较

黑士兵飞（Hermetia illucens），一种以捕食废物生物质为食并将其转化为有用的营养物质（如蛋白质，脂质和甲壳质）而闻名的昆虫，已在昆虫农场大规模饲养。在这项研究中，几丁质是使用不同的提取方案从黑士兵苍蝇的外骨骼中分离得到的，例如化学溶剂，酶辅助分馏和超临界二氧化碳提取。然后通过使用傅立叶变换红外光谱，元素分析和X射线衍射确定提取的昆虫几丁质的理化性质，评估使用不同提取方法回收几丁质级分的有效性水平。结果表明，使用生态友好型酸和溶剂，例如乳酸，乙酸，

更新日期：2021-05-22

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