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Immobilization of Fungal β-Galactosidase through Self-Assembly of Hierarchical Microflowers under Optimal Activity Conditions
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-03-12 , DOI: 10.1021/acsfoodscitech.1c00015
Elizabet Moreno-Reyes 1 , Julie M. Goddard 1
Affiliation  

Many enzymes whose optimal activities occur at neutral pH values have been successfully immobilized in biocatalytic nano- and microflowers; however, a need for research on the preparation of these structures with enzymes that perform better under acidic conditions typical of many food processing operations remains. Here we demonstrate the influence of pH (5.0, 6.0, and 7.0) and salt concentration (0 or 137 mM NaCl) on the synthesis of hierarchically structured biocatalytic microflowers using copper phosphate and fungal β-galactosidase. Microflowers (2.15–2.45 μm) were obtained at pH 5.0 without NaCl even though other conditions yielded varying morphologies. While pH affected immobilization yield and protein load, the absence of NaCl favorably altered the protein load and activity. At pH 5.0 without NaCl, microflowers had the highest protein load, and β-galactosidase retained considerable activity compared to its free counterpart. Such findings demonstrate the importance of conditions of the media on the preparation of biocatalytic microflowers and their performance in food processing applications.

中文翻译:

在最佳活性条件下通过分层微花的自组装固定真菌β-半乳糖苷酶

在中性pH值下具有最佳活性的许多酶已成功地固定在生物催化的纳米花和微型花中。然而,仍然需要研究用在许多食品加工操作中典型的酸性条件下表现更好的酶来制备这些结构的方法。在这里,我们证明了pH(5.0、6.0和7.0)和盐浓度(0或137 mM NaCl)对使用磷酸铜和真菌β-半乳糖苷酶合成具有层次结构的生物催化微花的影响。即使在其他条件下产生不同形态的条件下,在没有NaCl的pH 5.0的条件下也可获得微花(2.15-2.45μm)。pH值影响固定化产量和蛋白质负载,而NaCl的缺乏有利地改变了蛋白质负载和活性。在没有NaCl的pH 5.0下,微花的蛋白质负载量最高,与β-半乳糖苷酶相比,β-半乳糖苷酶保留了相当大的活​​性。这些发现证明了培养基条件对生物催化微花的制备及其在食品加工应用中的性能的重要性。
更新日期:2021-04-23
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