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A Highly Efficient Biocatalytic Conversion of Renewable Pectic Polysaccharide Biomass into Bioactive Oligogalacturonides
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-02-15 , DOI: 10.1021/acsfoodscitech.0c00040 Guojun Yang 1, 2, 3 , Shuguang Li 1 , Haidong Tan 1 , Kuikui Li 1 , Wei Chen 1 , Heng Yin 1
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-02-15 , DOI: 10.1021/acsfoodscitech.0c00040 Guojun Yang 1, 2, 3 , Shuguang Li 1 , Haidong Tan 1 , Kuikui Li 1 , Wei Chen 1 , Heng Yin 1
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The quest for an efficient biodegradation of pectic polysaccharides into sustainable oligogalacturonide (OG) products for a recent bioactivity study and application is currently underway. This study reported highly efficient degradation of pectin into OGs (DP2–DP10) by a polygalacturonase (i.e., AnPG28A from Aspergillus niger). The activity of AnPG28A toward low-methoxyl pectin reached 42897 units/mL, and the protein concentration was 7.7 g/L after 96 h, the highest of the reported values. For further processing of commercial pectins to produce OGs, the physicochemical properties of different kinds of commercial pectins on the market were characterized. The preparation of high-purity OG standards (DP2–DP7) was achieved with polygalacturonic acid as a substrate by high-performance liquid chromatography, which helps the preparation of OG, its usage, and the study of its functional characteristics. Moreover, further OG production pilot experiments were performed on the basis of a response surface methodology. The OG yield was approximately 65% on the pilot scale. AnPG28A has many advantages, such as easy, inexpensive preparation, greenness, and excellent catalytic activity. The high yield and quality of OGs and the sustainability and eco-friendly process support the forthcoming commercialization of the biocatalytic transformation of pectin into high-value-added OGs.
中文翻译：
可再生的果胶多糖生物质高效生物催化转化为生物活性寡半乳糖苷。
目前正在寻求将果胶多糖有效地生物降解为可持续的低聚半乳糖苷（OG）产品，以用于最近的生物活性研究和应用。这项研究报告说，聚半乳糖醛酸酶（即黑曲霉的PG28A ）可将果胶高效降解为OG（DP2-DP10 ）。An的活动PG28A朝向低甲氧基果胶的浓度达到42897单位/ mL，96小时后蛋白质浓度为7.7 g / L，为报道值的最高值。为了进一步加工商业果胶以生产OG，对市场上不同种类的商业果胶的理化性质进行了表征。以聚半乳糖醛酸为底物，通过高效液相色谱法完成了高纯度OG标准品（DP2-DP7）的制备，这有助于OG的制备，使用和功能特性的研究。此外，基于响应面方法进行了进一步的OG生产中试实验。OG产率约为中试规模的65％。一个PG28A具有许多优点，例如，简便，廉价的制备方法，绿色环保性和出色的催化活性。OG的高产量和高质量以及可持续性和环保工艺支持了果胶生物催化转化为高附加值OG的即将商业化。
更新日期：2021-04-23
中文翻译：
可再生的果胶多糖生物质高效生物催化转化为生物活性寡半乳糖苷。
目前正在寻求将果胶多糖有效地生物降解为可持续的低聚半乳糖苷（OG）产品，以用于最近的生物活性研究和应用。这项研究报告说，聚半乳糖醛酸酶（即黑曲霉的PG28A ）可将果胶高效降解为OG（DP2-DP10 ）。An的活动PG28A朝向低甲氧基果胶的浓度达到42897单位/ mL，96小时后蛋白质浓度为7.7 g / L，为报道值的最高值。为了进一步加工商业果胶以生产OG，对市场上不同种类的商业果胶的理化性质进行了表征。以聚半乳糖醛酸为底物，通过高效液相色谱法完成了高纯度OG标准品（DP2-DP7）的制备，这有助于OG的制备，使用和功能特性的研究。此外，基于响应面方法进行了进一步的OG生产中试实验。OG产率约为中试规模的65％。一个PG28A具有许多优点，例如，简便，廉价的制备方法，绿色环保性和出色的催化活性。OG的高产量和高质量以及可持续性和环保工艺支持了果胶生物催化转化为高附加值OG的即将商业化。
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