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Green synthesized AgNPs from Periploca hydaspidis Falc. and its biological activities
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-04-21 , DOI: 10.1002/jemt.23780
Saima Ali 1 , Muhammad Rashid Khan 1 , Raees Khan 2
Affiliation  

Characterization of bio-synthesized silver nanoparticles (AgNPs) using Periploca hydaspidis (PHAgNPs) whole plant extract for the first time via UV–Visible spectroscopy, XRD, FTIR, DLS, and SEM analysis techniques was done. A rich variety of phytochemicals in P. hydaspidis aqueous extract (PHA) functioned as possible reducing and capping agents for AgNPs synthesis. In vitro antioxidant activities (DPPH, Iron chelating, Hydroxyl ion, Nitric oxide, and β-carotene bleaching assays) of PHAgNPs revealed least IC50 values especially in hydroxyl ion (39.08 ± 0.88 μg/mL) and nitric oxide (37.53 ± 2.24 μg/mL) scavenging assays relative to standard controls (ascorbic acid, rutin, and gallic acid) and PHA. In addition, visible inhibition zone diameters were formed around discs against all pathogenic microbial strains including multi-drug resistant strains (MDR's). MIC and MBC/MFC were depicted least in PHAgNPs with maximum bactericidal/fungicidal effects. MTT assay displayed a significant antiproliferative potential of PHAgNPs against HCCLM3, MCF-7, MDA-MB 231, and HEPG2 cancer cell lines, where least IC50 values were recorded against HEPG2 (12.97 ± 0.04 μg/mL) and MCF-7 (5.73 ± 0.22 μg/mL). Furthermore, PHAgNPs considerably (p > 0.001) prevented the migration of MCF-7 cancer cells in vitro whereas in in vivo wound healing assay, faster skin regeneration, and epithelization in wound biopsies was observed via histological analysis. PHAgNPs treated group rats significantly increased (p < 0.05) the wound contraction rate, hydroxyproline content and hemostatic potential compared to control and PHA-treated groups.

中文翻译:

Green 合成了来自 Periploca hydaspidis Falc 的 AgNPs。及其生物活性

首次通过紫外-可见光谱、XRD、FTIR、DLS 和 SEM 分析技术,使用Periploca hydaspidis (PHAgNPs) 全植物提取物对生物合成的银纳米粒子 (AgNPs) 进行表征。P. hydaspidis水提取物 (PHA) 中丰富多样的植物化学物质可作为 AgNPs 合成的可能还原剂和封端剂。PHAgNPs 的体外抗氧化活性(DPPH、铁螯合、羟基离子、一氧化氮和 β-胡萝卜素漂白试验)显示 IC 50最低与标准对照(抗坏血酸、芦丁和没食子酸)和 PHA 相关的氢氧根离子 (39.08 ± 0.88 μg/mL) 和一氧化氮 (37.53 ± 2.24 μg/mL) 清除测定中的值。此外,在圆盘周围形成了可见的抑菌圈直径,以抵抗所有致病微生物菌株,包括多重耐药菌株 (MDR)。MIC 和 MBC/MFC 在 PHAgNPs 中描述最少,具有最大的杀菌/杀真菌作用。MTT测定显示针对HCCLM3,MCF-7,MDA-MB 231,和HEPG2癌细胞系,其中至少IC PHAgNPs的显著抗增殖潜力50值被记录针对HEPG2(12.97±0.04微克/毫升)和MCF-7(5.73 ± 0.22 微克/毫升)。此外,PHAgNPs 相当多(p > 0.001) 在体外阻止 MCF-7 癌细胞的迁移,而在体内伤口愈合试验中,通过组织学分析观察到更快的皮肤再生和伤口活检中的上皮形成。与对照组 和 PHA 治疗组相比,PHAgNPs 治疗组大鼠的伤口收缩率、羟脯氨酸含量和止血潜力显着增加(p < 0.05)。
更新日期:2021-04-21
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