具有巨大的含钼多金属氧酸盐的HPV衣壳蛋白L1杂合蛋白可提高病毒样颗粒的稳定性和[Mo154]的抗肿瘤作用,Biomaterials Science

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具有巨大的含钼多金属氧酸盐的HPV衣壳蛋白L1杂合蛋白可提高病毒样颗粒的稳定性和[Mo154]的抗肿瘤作用
Biomaterials Science ( IF 6.6 ) Pub Date : 2021-4-13 , DOI: 10.1039/d1bm00138h
Ya-Rong Xue _{1,

2,

3,

4,

5} , Yu Wang _{1,

2,

3,

4,

5} , Gang Chen _{1,

2,

3,

4,

5} , Bo Sun _{4,

5,

6,

7,

8} , Bao Li _{1,

2,

3,

4,

5} , Lixin Wu _{1,

2,

3,

4,

5} , Yuqing Wu _{1,

2,

3,

4,

5}

Affiliation

我们报告了一种生物-无机杂交系统，[Mo ₁₅₄ ] @VLPs，由HPV衣壳蛋白L1的病毒样颗粒（VLP）和[Mo ₁₅₄ ]的巨大盘状，含钼多金属氧酸盐构成。通过CsCl梯度离心纯化杂合体，并进一步通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），动态光散射（DLS）和透射电子显微镜（TEM）进行验证。具有[Mo ₁₅₄ ]的组件提高了VLP对pH，温度和储存时间的耐受性，从而为降低HPV疫苗的成本提供了机会。此外，[Mo ₁₅₄被包裹在VLP中后，杀死癌细胞的杀伤力提高了6％，这主要归因于[Mo ₁₅₄ ]的增强的生物相容性。[Mo ₁₅₄ ]和[Mo ₁₅₄ ] @VLP都被808 nm的红外光照射，分别将其破坏癌细胞的能力提高了3倍和2倍，证实了[Mo ₁₅₄ ]是一种有效的抗肿瘤光热剂。因此，L1-p与[Mo ₁₅₄ ]的成功杂交提高了VLP的稳定性，同时为增强[Mo ₁₅₄ ]的抗肿瘤能力铺平了道路，并进一步扩展了其作为未来抗肿瘤药物的应用前景。

"点击查看英文标题和摘要"

A hybrid HPV capsid protein L1 with giant Mo-containing polyoxometalate improves the stability of virus-like particles and the anti-tumor effect of [Mo154]

We report a bio-inorganic hybrid system, [Mo₁₅₄]@VLPs, constructed from the virus-like particles (VLPs) of the HPV capsid protein L1 and a giant disc-shaped, molybdenum-containing polyoxometalate of [Mo₁₅₄]. The hybrid was purified by CsCl gradient centrifugation and further validated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS) and transmission electron microscopy (TEM). An assembly with [Mo₁₅₄] improved the tolerance of VLPs to pH, temperature, and storage time, thereby defining an opportunity to reduce the cost of HPV vaccines. Moreover, the ability of [Mo₁₅₄] to kill cancer cells was improved by 6% after being encapsulated inside the VLPs, which is mainly attributed to the enhanced biocompatibility of [Mo₁₅₄]. The irradiation of both [Mo₁₅₄] and [Mo₁₅₄]@VLPs with an infrared light of 808 nm further enhanced their ability to destroy cancer cells by 3- and 2-fold, respectively, confirming that [Mo₁₅₄] is an effective anti-tumor photo-thermal agent. Therefore, the successful hybrid of L1-p and [Mo₁₅₄] improves the stability of VLPs and simultaneously paves the way to enhance the anti-tumor ability of [Mo₁₅₄] and further extends its application prospects as a future anti-tumor drug.

更新日期：2021-04-23

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