Berberis aristata DC., well known as ‘Indian barberry’ or ‘tree turmeric’, (Berberidaceae) is a medicinal herb native to the Northern Himalaya. A dig in the research literature evinced different pharmacological and therapeutic effects of B. aristata and its isoquinoline alkaloids. Records on investigations for the chemical composition of the plant corroborated isoquinoline alkaloids, berberine, berbamine, palmatine, magnoflorine and jatrorrhizine as the important bioactive constituents. Recent upsurge in the demand of botanicals is a result of their scientific validation for efficacy and quality. Keeping this in view, a validated simultaneous high-performance thin-layer chromatography (HPTLC) method has been developed using silica gel 60 F₂₅₄ as the stationary phase and ethyl acetate‒formic acid‒glacial acetic acid‒water (100:11:11:26, V/V) as the mobile phase. The plates were scanned densitometrically at 254 nm and 366 nm. The limits of detection and the percentage mean recoveries were 323.24, 227.00, 55.68, 88.67 and 86.56 ng/spot, and 99.35%, 99.98%, 98.55%, 99.40% and 98.92%, for berberine, berbamine, palmatine, magnoflorine and jatrorrhizine, respectively. The linearity range for berberine and berbamine was 1000‒6000 ng/spot, for palmatine 200‒700 ng/spot, for magnoflorine 500‒2000 ng/spot and for jatrorrhizine 300‒1800 ng/spot. The proposed HPTLC method offers a sensitive, specific and precise gage for the concurrent estimation of these five representative biomarkers of B. aristata and also aids in its qualitative analysis, since a fingerprint is generated, wherein the five active ingredients are resolved with other compounds present in the root.

小ber（Berberis aristata DC。），俗称“印度伏牛花”或“姜黄树”（小er科），是喜马拉雅北部的药用植物。研究文献中的研究表明，牛油树及其异喹啉生物碱具有不同的药理和治疗作用。关于植物化学成分的调查记录，证实了其为重要的生物活性成分，包括异喹啉碱，小ber碱，贝巴明，棕榈碱，厚朴碱和麻风树碱。最近对植物药的需求激增是由于其对功效和质量的科学验证所致。考虑到这一点，使用硅胶60 F ₂₅₄开发了一种经过验证的同时高效薄层色谱（HPTLC）方法作为固定相，乙酸乙酯，甲酸，冰醋酸，水（100：11：11：26，V / V）作为流动相。在254nm和366nm处光密度扫描板。小ber碱，贝巴明，巴马汀，厚朴碱和风麻草碱的检出限和平均回收率分别为323.24、227.00、55.68、88.67和86.56 ng / spot，以及99.35％，99.98％，98.55％，99.40％和98.92％。分别。小ber碱和贝巴明的线性范围为1000‒6000 ng /点，巴马汀200‒700 ng /点，木兰花素500‒2000 ng /点，麻风树碱300‒1800 ng /点。所提出的HPTLC方法为同时评估这两种代表性的B. aristata生物标志物提供了灵敏，特异性和精确的量具 由于生成了指纹，其中的五种活性成分与根中存在的其他化合物分离，因此还有助于其定性分析。