Fish-borne zoonotic trematodes (FBZT) are extremely important zoonotically and can infect humans via the consumption of poorly cooked fish containing active metacercariae. In this context, the present study aimed to update the epidemiological information of FBZT among Nile tilapia (Oreochromis niloticus) collected from Giza and Fayoum Governorates. Concerning the molecular characterization and phylogenetic analysis of adult flukes from experimentally infected pigeons and histopathological alterations of both larval and adult trematode flukes. Oreochromis niloticus were found to be infected with different encysted metacercaria (EMC); Prohemistomum, Haplorchis and Pygidiopsis species from wild caught in Giza and farmed fish in Fayoum with the total mean of prevalence that 81.89 ± 6.48, 18.03 ± 2.9 and 34.64 ± 3.42 respectively. Three recovered flukes from experimentally infected domestic pigeons (Columba livia domestica); Prohemistomum vivax, Haplorchis pumilio and Pygidiopsis genata in their small intestine. First molecular characterization and phylogenetic analysis of heterophyid flukes; P. genata and H. pumilio. The sequences obtained in this study were registered in the GenBank with accession numbers (MT672308.1 and MT707975.1) respectively. Moreover, constructing a phylogeny and phylogenetic relationships between two heterophyid species was performed through analytic study of the second internal transcribed spacer nuclear ribosomal genes (rDNA-ITS2). Phylogenetic analysis of P. genata and H. pumilio showed 99.42% nucleotide similarity with that sequence from Israel (AY245710) and 99.71% from Vietnam (EU826636.1). In addition, histopathological alterations of EMC and adult flukes induced necrosis of fish muscle bundles and a severe inflammatory response with muscular necrosis in intestinal tract of infected pigeons.

中文翻译：

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感染尼罗罗非鱼的鱼源性人畜共患吸虫的流行病学研究，首次对两种异食吸虫进行分子鉴定

鱼源性人畜共患吸虫 (FBZT) 在人畜共患病方面极为重要，可通过食用含有活性包蚴的未煮熟的鱼来感染人类。在此背景下，本研究旨在更新从吉萨省和法尤姆省收集的尼罗罗非鱼（Oreochromis niloticus）中 FBZT 的流行病学信息。关于来自实验感染的鸽子的成年吸虫的分子特征和系统发育分析以及吸虫幼虫和成年吸虫吸虫的组织病理学改变。Oreochromis niloticus被发现感染了不同的包囊囊蚴（EMC）；Prohemistomum , Haplorchis和Pygidiopsis来自吉萨野生捕获的物种和法尤姆养殖鱼类的总流行率平均值分别为 81.89 ± 6.48、18.03 ± 2.9 和 34.64 ± 3.42。从实验感染的家鸽 ( Columba liviadomestica ) 中回收了三只吸虫；Prohemistomum vivax、Haplorchis pumilio和Pygidiopsis genata在它们的小肠中。第一次异型吸虫的分子特征和系统发育分析；P。 genata和H . 普米利奥. 本研究中获得的序列分别以登录号（MT672308.1和MT707975.1）在GenBank中注册。此外，通过对第二个内部转录间隔核核糖体基因（rDNA-ITS2）的分析研究，构建了两个异类物种之间的系统发育和系统发育关系。P 的系统发育分析。 genata和H . pumilio与来自以色列 (AY245710) 的序列和来自越南 (EU826636.1) 的序列具有 99.42% 的核苷酸相似性。此外，EMC 和成年吸虫的组织病理学改变导致鱼肌肉束坏死和严重的炎症反应，感染鸽子的肠道肌肉坏死。