Selenitetriglycerides are biologically active, organic forms of selenium formed as a result of the modification of selenic acid and sunflower oil. Studies in rats have shown that they are well absorbed and of low toxicity. There are no published studies on selenitetriglycerides supplementation in calves. In this study, selenitetriglycerides were administered once orally on the 2nd day of life at a dose of 0.5 or 1 mg Se/kg body weight to each of six Holstein-Friesian calves while six control calves were not supplemented. Blood for determination of selenium concentration, glutathione peroxidase activity, haematological parameters, aspartate aminotransferase, creatine kinase, and lactate dehydrogenase activities and glucose, total protein, albumin, triglycerides, cholesterol, urea, and creatinine concentration was collected before supplementation (day 0) and 1, 2, 5, 10 and 14 days after supplementation. Selenitetriglycerides administration increased (P < 0.01) serum selenium concentration in supplemented calves as early as day1, from a mean of 63.4 to 184.22 µg/l in calves receiving selenium at a dose of 0.5 mg/kg BW, and from 63.17 to 200.33 µg/l in calves receiving 1 mg/kg. Serum selenium concentrations remained significantly higher compared to the control group throughout the experiment. Glutathione peroxidase activity was higher in supplemented than control calves, significantly so in animals receiving the 1 mg/kg dose of Se on the 10th and 14th days (P < 0.05). There were no significant differences in the haematological and biochemical parameters between the groups. This experiment showed that supplementation with selenitetriglycerides could significantly improve blood selenium status in calves without adverse effects on haematological or biochemical parameters. These findings are essential prerequisites for future studies on selenitetriglycerides supplementation to manage clinical selenium deficiency in calves.

中文翻译：

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两种剂量率下单次口服亚硒酸甘油三酯对荷斯坦-弗里斯兰犊牛血硒状态以及血液学和生化参数的影响

亚硒酸甘油三酯是硒酸和葵花籽油改性后形成的具有生物活性的有机形式的硒。对大鼠的研究表明它们吸收良好且毒性低。目前还没有发表关于补充犊牛亚硒酸甘油三酯的研究。在这项研究中，六头荷斯坦-弗里斯兰小牛在出生后第二天口服一次亚硒酸三甘油酯，剂量为每公斤体重 0.5 或 1 毫克硒，而六头对照小牛没有补充。在补充前（第0天）和收集血液用于测定硒浓度、谷胱甘肽过氧化物酶活性、血液学参数、天冬氨酸转氨酶、肌酸激酶和乳酸脱氢酶活性以及葡萄糖、总蛋白、白蛋白、甘油三酯、胆固醇、尿素和肌酸酐浓度。补充后1、2、5、10和14天。早在第一天，添加亚硒酸甘油三酯即可增加（P < 0.01）补充犊牛的血清硒浓度，以 0.5 mg/kg BW 剂量接受硒的犊牛血清硒浓度从平均 63.4 µg/l 增加至 184.22 µg/l，并从 63.17 µg/l 增加至 200.33 µg/l。 l 接受 1 mg/kg 剂量的小牛。在整个实验过程中，与对照组相比，血清硒浓度仍然显着较高。补充后的小牛中谷胱甘肽过氧化物酶活性高于对照小牛，在第 10 天和第 14 天接受 1 mg/kg 剂量硒的动物中亦是如此（P < 0.05）。各组之间的血液学和生化参数没有显着差异。该实验表明，补充亚硒酸甘油三酯可以显着改善犊牛的血硒状况，且不会对血液学或生化参数产生不利影响。这些发现是未来研究补充亚硒酸甘油三酯以控制犊牛临床硒缺乏症的必要先决条件。