ABSTRACT

Understanding how strawberry (Fragaria x ananassa) nutritional and quality traits are genetically regulated and correlated is an essential step towards improving marker-assisted breeding programmes in this crop. A first step to achieve this goal was to construct a single nucleotide polymorphism (SNP)-based genetic map of 140 F₁ individuals of a cross between the 2 octoploid parents ‘Redgauntlet’ and ‘Hapil’. The map consisted of 3933 SNPs distributed over 28 linkage groups, representing the 7 homoeologous groups expected in Fragaria (2n = 8x = 56), and covered a total length of 2624.7 cM with an average resolution of 0.7 cM/SNP. Two overlapping subsets of F₁ individuals were evaluated in the field and glasshouse at East Malling Research and the University of Reading, respectively. Negative correlation was observed between Pelargonidin and the three traits L*, a*, and b*, whereas positive correlations were observed within L*, a*, and b*, and between Ellagic acid, Pelargonidin, and Cyanidin at both trial locations. Quantitative trait loci (QTL) mapping revealed 29 significant QTL associated with the measured traits and mapped over 16 linkage groups. Two QTL for fruit fresh weight were co-located between day 1 and day 4 of shelf life, accounting for over 62% of the variation. These data will enhance our understanding of the genetic basis and correlation of strawberry quality traits and provide the basis for refining QTL that underpin the genetic regulation of these quality traits.

摘要

了解草莓 ( Fragaria x ananassa ) 的营养和品质性状如何受到遗传调控和关联，是改进该作物标记辅助育种计划的重要一步。第一步骤为实现这一目标是构建一个单核苷酸多态性（SNP）为基础的140°F的遗传图谱₁ 2个八倍体父母Redgauntlet'和“Hapil”之间的交叉的个人。该图由分布在 28 个连锁群上的 3933 个 SNP 组成，代表草莓中预期的 7 个同源群(2n = 8x = 56)，总长度为 2624.7 cM，平均分辨率为 0.7 cM/SNP。F _{1 的}两个重叠子集个体分别在 East Malling Research 和雷丁大学的野外和温室中进行评估。在天竺葵素与 L*、a* 和 b* 三个性状之间观察到负相关，而在两个试验地点的 L*、a* 和 b* 内以及鞣花酸、天竺葵素和花青素之间观察到正相关。数量性状位点 (QTL) 定位揭示了与测量的性状相关的 29 个显着 QTL，并定位了 16 个连锁群。水果鲜重的两个 QTL 位于保质期的第 1 天和第 4 天之间，占变异的 62% 以上。这些数据将增强我们对草莓品质性状遗传基础和相关性的理解，并为完善支撑这些品质性状遗传调控的QTL提供基础。