ABSTRACT

Our aim was to screen new hyper-variable mango SSRs (MSSRs) designed from Amrapali genome sequences to find polymorphic ones between Amrapali and Sensation parental genotypes. Out of 253 MSSRs screened against Amrapali and Sensation genotypes, 212 were polymorphic, of which 8 MSSRs showed aa, bb or bb, aa allelic patterns. MSSRs showing polymorphism in terms of homozygous for ‘a’ allele in Amrapali and homozygous ‘b’ allele for Sensation and vice versa were selected for assignment of parentage of hand-pollinated (Amrapali x Sensation) hybrids and open-pollinated Amrapali. MSSR markers were mapped back to the Amrapali genome version 3.0 NCBI GenBank database. MSSRs (M109, P125, M15, and M73) assigned parentage to more than 88.0% of hand-pollinated hybrids. P125 confirmed parentage of 93.61% progenies followed by M109 (90.43%), M15 (89.36%), and M73 (88.29%). Randomly three MSSRs, viz. M109, P125, and M73, were used for assignment of parentage of OP seedlings of Amrapali. Out of 22 OP seedlings, only 2 seedlings showed the identical heterozygous allelic pattern and their hybrid origin was confirmed. It is concluded that MSSRs (P125, M109, M15, and M73) from the Amrapali genome have immense value in MAS in mango and can be utilised for early stage selection of true hybrids.

摘要

我们的目标是筛选从 Amrapali 基因组序列设计的新的超变芒果 SSR (MSSR)，以找到 Amrapali 和 Sensation 亲本基因型之间的多态性。在针对 Amrapali 和 Sensation 基因型筛选的 253 个 MSSR 中，212 个是多态的，其中 8 个 MSSR 显示 aa、bb 或 bb、aa 等位基因模式。选择在 Amrapali 中“a”等位基因纯合子和 Sensation 纯合子“b”等位基因多态性的 MSSR，反之亦然，用于分配手工授粉 (Amrapali x Sensation) 杂种和开放授粉 Amrapali 的亲本。MSSR 标记被映射回 Amrapali 基因组 3.0 版 NCBI GenBank 数据库。MSSR（M109、P125、M15 和 M73）将亲本分配给超过 88.0% 的手工授粉杂交种。P125 确认了 93.61% 后代的亲子关系，其次是 M109 (90.43%)、M15 (89.36%)、和 M73 (88.29%)。随机三个 MSSR，即。M109、P125 和 M73 用于分配 Amrapali OP 幼苗的亲子关系。在 22 株 OP 幼苗中，只有 2 株幼苗显示出相同的杂合等位基因模式，并且证实了它们的杂交来源。结论是，来自 Amrapali 基因组的 MSSR（P125、M109、M15 和 M73）在芒果的 MAS 中具有巨大价值，可用于真正杂种的早期选择。