Severe fever with thrombocytopenia syndrome virus (SFTSV) is newly discovered virus which is the member of the order Bunyavirales, family phenuiviridae, phlebovirus genus. Its genome is composed of 3 segments of negative-sense RNA L, M and S. NSs is a non structure protein encoded by S segment which is important for viral replication and virulence. NSs protein of SFTSV is only involved in the regulation of host innate immune responses and suppression of IFN-promoter activities. So, the exact functions of this protein need to be studied deeply. To understand the exact role of NSs from SFTSV in viral replication and host immune response, a qualified antibody against this protein is required. In this study, NSs gene of SFTSV, was cloned into a bacterial expression vector (pGEX-6P-1) and the recombinant plasmid was transformed into Escherichia coli BL21 (DE3) cells. The SFTSV NSs fusion protein was purified using Glutathione Sepharose 4B and utilized as an antigen to immunize rabbits and obtain an anti-SFTSV NSs polyclonal antibody. Proper expression of the fusion protein and polyclonal antibody specificity were confirmed by western blotting and immunofluorescence analyses. The polyclonal antibody recognized NSs from SFTSV specifically. This is the first report that NSs can form viroplasm-like structures not only in infected cells but also in transfected cells with NSs plasmids. This polyclonal antibody will be useful for future studies of NSs functions.

重症发热伴血小板减少综合征病毒 (SFTSV) 是新发现的病毒，属于布尼亚病毒目、嗜热病毒科、静脉病毒科的成员属。其基因组由 3 个负义 RNA L、M 和 S 片段组成。NSs 是由 S 片段编码的非结构蛋白，对病毒复制和毒力很重要。SFTSV 的 NSs 蛋白仅参与宿主先天免疫反应的调节和干扰素启动子活性的抑制。因此，需要深入研究这种蛋白质的确切功能。要了解来自 SFTSV 的 NSs 在病毒复制和宿主免疫反应中的确切作用，需要针对该蛋白质的合格抗体。本研究将SFTSV的NSs基因克隆到细菌表达载体（pGEX-6P-1）中，并将重组质粒转化大肠杆菌BL21 (DE3) 细胞。用Glutathione Sepharose 4B纯化SFTSV NSs融合蛋白，作为抗原免疫家兔，获得抗SFTSV NSs多克隆抗体。通过蛋白质印迹和免疫荧光分析证实了融合蛋白的正确表达和多克隆抗体特异性。多克隆抗体特异性识别来自SFTSV的NS。这是首次报道 NSs 不仅可以在受感染的细胞中而且可以在用 NSs 质粒转染的细胞中形成病毒质样结构。这种多克隆抗体将有助于 NSs 功能的未来研究。